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微信小章| 产品名称： | HCC1419 |
|---|---|
| 商品货号： | TS212067 |
| Organism： | Homo sapiens, human |
| Tissue： | mammary gland; breast |
| Cell Type： | Epithelial |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent, The line grows as large epithelial attached cells in island-like formation. |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | TNM stage IIIA, grade 3,primary ductal carcinoma |
| Age： | 42 years |
| Gender： | female |
| Ethnicity： | Hispanic |
| Storage Conditions： | liquid nitrogen vapor temperature |
| Karyotype： | aneuploid |
| Derivation： | This cell line was initiated on 12/28/94 from a patient who received prior chemotherapy with no previous family history of breast cancer. The line took 9 months to establish. |
| Clinical Data： | female
Hispanic
42 years |
| Receptor Expression： | estrogen receptor, negative progesterone receptor, negative |
| Oncogene： | her2/neu +, p53 - |
| Genes Expressed： | Epithelial glycoprotein 2 EGP2; cytokeratin 19 |
| Cellular Products： | Epithelial glycoprotein 2 EGP2; cytokeratin 19 |
| Comments： | The tumor was classified as TNM stage IIIA, grade 3, invasive ductal carcinoma with 5 out of 5 lymph node metastasis.xa0 The cells are poorly differentiated.xa0 The cells overexpress Her2-neu but are negative for expression of p53. HCC1419 is positive for the epithelial cell specific marker epithelial glycoprotein 2 (EGP2) and for cytokeratin 19.xa0 The cells are negative for expression of estrogen receptor (ER) and for expression of progesterone receptor (PR).
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| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
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| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Medium Renewal: 2 to 3 times a week. Note:xa0The line grows as large epithelial attached cells in island-like formation. Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 13 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney,xa05th edition, published byxa0Wiley-Liss, N.Y., 2005. |
| Cryopreservation： | Freeze medium: Complete growth medium 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor temperature |
| Culture Conditions： | Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C |
| STR Profile： | Amelogenin: X CSF1PO: 10,12 D13S317: 9,12 D16S539: 11,12 D5S818: 10,12 D7S820: 11 THO1: 6,9.3 TPOX: 8 vWA: 16,17 |
| Name of Depositor： | AF Gazdar, AK Virmani |
| Year of Origin： | December 28, 1994 |
| References： | Gazdar AF, et al. Characterization of paired tumor and non-tumor cell lines established from patients with breast cancer. Int. J. Cancer 78: 766-774, 1998. PubMed: 9833771 |