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微信小章| 产品名称： | H4-II-E-C3 |
|---|---|
| 商品货号： | TS212068 |
| Organism： | Rattus norvegicus, rat |
| Tissue： | liver |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | hepatoma |
| Gender： | male |
| Strain： | AxC |
| Storage Conditions： | liquid nitrogen vapor phase |
| Clinical Data： | male |
| Genes Expressed： | tyrosine aminotransferase; albumin; transferrin; prothrombin |
| Cellular Products： | tyrosine aminotransferase; albumin; transferrin; prothrombin |
| Tumorigenic： | Yes |
| Effects： | Yes, in AxC rats |
| Comments： | Tyrosine amino transferase is inducible with glucorticoids, insulin or cAMP derivatives. The cells are productively infected with a retrovirus. |
| Complete Growth Medium： | Dulbeccos modified Eagles medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose, 75%; horse serum, 20%; fetal bovine serum, 5%.
|
| Subculturing： | Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:12 is recommended
Medium Renewal: Every 2 to 3 days |
| Cryopreservation： | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Temperature: 37°C |
| Name of Depositor： | JE Becker |
| Deposited As： | Rattus sp. |
| References： | Potter VR, Morse PA Jr.. Pyrimidine metabolism in tissue culture cells derived from rat hepatomas. I. Suspension cell cultures derived from the Novikoff hepatoma. Cancer Res. 25: 499-508, 1965. PubMed: 14297488 Reuber MD. A transplantable bile-secreting hepatocellular carcinoma in the rat. J. Natl. Cancer Inst. 26: 891-899, 1961. PubMed: 13740982 Weinstein IB, et al. Type C virus from cell cultures of chemically induced rat hepatomas. Science 178: 1098-1100, 1972. PubMed: 4343844 Klemm DJ, et al. Adenovirus E1A proteins regulate phosphoenolpyruvate carboxykinase gene transcription through multiple mechanisms. J. Biol. Chem. 271: 8082-8088, 1996. PubMed: 8626493 Peraino C, et al. Hepatomas in tissue culture compared with adapting liver in vivo. Natl. Cancer Inst. Monogr. 13: 229-245, 1964. PubMed: 14143233 |
| Cross References： | Nucleotide (GenBank) : AJ010024 Rattus norvegicus mRNA for Mi-2 autoantigen, partial. |