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| 产品名称： | H9/HTLV-IIIB |
|---|---|
| 商品货号： | TS212071 |
| Organism： | Homo sapiens, human |
| Cell Type： | T lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 2 Cells Contain RETROVIRUS
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | The cells produce HTLV III. |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Genes Expressed： | Human T cell leukemia virus III (HTLV III); Human Immunodeficiency virus (HIV) |
| Cellular Products： | Human T cell leukemia virus III (HTLV III); Human Immunodeficiency virus (HIV) |
| Comments： | The cells produce HTLV III. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing： | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Culture Conditions： | Temperature: 37.0°C |
| Name of Depositor： | The United States of America |
| U.S. Patent Number： | |
| References： | Popovic M, et al. Serological detection of antibodies to HTLV-III in sera of patients with AIDS and pre-AIDS conditions. US Patent 4,520,113 dated May 28 1985 Chang SY, et al. The origin of HIV-1 isolate HTLV-IIIB. Nature 363: 466-469, 1993. PubMed: 8502298 Trkola A, et al. Human momoclonal antibody 2G12 defines a distinctive neutralization epitope on the gp120 glycoprotein of human immunodeficiency virus type 1. J. Virol. 70: 1100-1108, 1996. PubMed: 8551569 |