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微信小章| 产品名称： | H-4-II-E |
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| 商品货号： | TS212072 |
| Organism： | Rattus norvegicus, rat |
| Tissue： | liver |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | hepatoma |
| Applications： | The line is useful for detecting picogram quantities of planar polychlorinated organic compounds in environmental samples and food extracts. |
| Genes Expressed： | xad |
| Cellular Products： | aryl hydrocarbon hydroxylase is inducible |
| Comments： | The line has been cured of Mycoplasma arginini contamination.
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| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended Medium Renewal: Every 2 to 3 days |
| Cryopreservation： | culture medium 95%; DMSO, 5% |
| Culture Conditions： | Temperature: 37°C |
| Name of Depositor： | JA Bradlaw |
| Deposited As： | Rattus sp. |
| U.S. Patent Number： | |
| References： | Benedict WF, et al. Aryl hydrocarbon hydroxylase induction in mammalian cell culture--IV. Stimulation of the enzyme activity in established cell lines derived from rat or mouse hepatoma and from normal rat liver. Biochem. Pharmacol. 22: 2766-2769, 1973. PubMed: 4357894 . . In Vitro 16: 230, 1980. Sassier P, Bergeron M. Specific inhibition of cell proliferation in the mouse intestine by an aqueous extract of rabbit small intestine. Cell Tissue Kinet. 10: 223-243, 1977. PubMed: 872181 Bradlaw JA, Casterline JL Jr.. Induction of enzyme activity in cell culture: a rapid screen for detection of planar polychlorinated organic compounds. J. Assoc. Off. Anal. Chem. 62: 904-916, 1979. PubMed: 115836 Peraino C, et al. Hepatomas in tissue culture compared with adapting liver in vivo. Natl. Cancer Inst. Monogr. 13: 229-245, 1964. PubMed: 14143233 |
| Cross References： | Nucleotide (GenBank) : U49128 Rattus norvegicus neuroendocrine calcium channel alpha-1 subunit mRNA, variant CaCh3b (delta part IVS4), partial cds. Nucleotide (GenBank) : U49126 Rattus norvegicus neuroendocrine calcium channel alpha-1 subunit mRNA, variant CaCh3 (delta IVS3 delta IVS4), partial cds. Nucleotide (GenBank) : U49127 Rattus norvegicus neuroendocrine calcium channel alpha-1 subunit mRNA, variant CaCh3 (delta IVS3 delta part IVS4), partial cds. |