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微信小章| 产品名称： | H9c2(2-1) |
|---|---|
| 商品货号： | TS212077 |
| Organism： | Rattus norvegicus, rat |
| Tissue： | heart/myocardium |
| Cell Type： | Myoblast |
| Product Format： | frozen |
| Morphology： | myoblast |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age： | embryo |
| Strain： | BD1X |
| Applications： | This cell line is a suitable transfection host. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | H9c2(2-1) is a subclone of the original clonal cell line derived from embryonic BD1X rat heart tissue by B. Kimes and B. Brandt and exhibits many of the properties of skeletal muscle. |
| Receptor Expression： | acetylcholine, expressed |
| Genes Expressed： | myokinase; creatine phosphokinase; myosin |
| Cellular Products： | myokinase; creatine phosphokinase; myosin |
| Comments： | Myoblastic cells in this line will fuse to form multinucleated myotubes and respond to acetylcholine stimulation. Fusion occurs faster if the serum concentration in the medium is reduced to one percent. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.xa0Corning® T-75 flasks (catalog #430641) are recommended for subculturing this product.
The myoblastic population will become depleted rapidly if the cultures are allowed to become confluent.
To prevent loss of myoblastic cells, cultures should be subcultured before they become confluent, and the line should be recloned periodically with selection for myoblastic cells.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: Every 2 to 3 days |
| Cryopreservation： | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C |
| Name of Depositor： | W Carlisle |
| Deposited As： | Rattus sp. |
| References： | Kimes BW, Brandt BL. Properties of a clonal muscle cell line from rat heart. Exp. Cell Res. 98: 367-381, 1976. PubMed: 943302 Levy AP, et al. Post-transcriptional regulation of vascular endothelial growth factor by hypoxia. J. Biol. Chem. 271: 2746-2753, 1996. PubMed: 8576250 |