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微信小章| 产品名称： | H36.12j Pixie 12j |
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| 商品货号： | TS212086 |
| Organism： | Mus musculus (macrophage tumor cell line); Mus musculus (peritoneal macrophage), mouse(macrophage tumor cell line); mouse (peritoneal macrophage) |
| Cell Type： | Macrophage |
| Product Format： | frozen |
| Morphology： | macrophage |
| Culture Properties： | suspension (some adherent cells) |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | This cell line was established by P.A. Campbell and E.P. Canono in 1991. Percoll gradient purified, proteose peptone elicited peritoneal macrophage cells from C57BL/6N mice were fused with drug selected P388D1 mouse macrophage tumor cells. The cells produce tumor necrosis factor alpha (TNF alpha) upon direct beryllium stimulation and serve as an in vitro model for chronic beryllium disease in humans. Since intracellular Listeria escape into the cytoplasm where they replicate, the cell line is a useful tool in the study of the permissive intracellular growth cycle of Listeria. These cells may be used in assays for the phagocytosis and killing of microorganisms, in assays for the production of cytokines by mouse macrophages, and for assays for the effect of environmental toxins on mouse macrophages. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | This cell line was established by P.A. Campbell and E.P. Canono in 1991. Percoll gradient purified, proteose peptone elicited peritoneal macrophage cells from C57BL/6N mice were fused with drug selected P388D1 mouse macrophage tumor cells. |
| Genes Expressed： | surface Interleukin-10 (Interleukin 10; IL-10),tumor necrosis factor alpha (TNF alpha) upon direct beryllium stimulation |
| Cellular Products： | surface Interleukin-10 (Interleukin 10; IL-10) tumor necrosis factor alpha (TNF alpha) upon direct beryllium stimulation |
| Comments： | This cell line was established by P.A. Campbell and E.P. Canono in 1991. Percoll gradient purified, proteose peptone elicited peritoneal macrophage cells from C57BL/6N mice were fused with drug selected P388D1 mouse macrophage tumor cells. H36.12j is a model for the permissive intracellular growth of Listeria monocytogenes within mouse macrophages. The cells phagocytose Listeria via receptors for internalin A (InlA), a Listeria surface polypeptide. The cells produce tumor necrosis factor alpha (TNF alpha) upon direct beryllium stimulation and serve as an in vitro model for chronic beryllium disease in humans. Since intracellular Listeria escape into the cytoplasm where they replicate, the cell line is a useful tool in the study of the permissive intracellular growth cycle of Listeria. The H36.12j cells have been found to express biologically active surface IL-10 that regulates macrophage bacterial activity. These cells may be used in assays for the phagocytosis and killing of microorganisms, in assays for the production of cytokines by mouse macrophages, and for assays for the effect of environmental toxins on mouse macrophages. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002.
To make the complete growth medium, add the following components to the base medium:
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| Subculturing： | Add fresh medium every 2 to 3 days (depending on cell density). Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 x 105 viable cells/mL. Maintain cell density between 1 x 105 and 1 x 106 viable cells/mL. Adherent cells may be harvested by scraping. Medium Renewal: Every 2 to 3 days |
| Cryopreservation： | Complete growth medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| Name of Depositor： | RT Sawyer |
| Deposited As： | mouse |
| Year of Origin： | 1991 |
| References： | Canono EP, Campbell PA. Production of mouse inflammatory hybridomas. J. Tissue Culture Methods 14: 3-8, 1992. Fleming SD, Campbell PA. Macrophages have cell surface IL-10 that regulates macrophage bactericidal activity. J. Immunol. 56: 1143-1150, 1996. PubMed: 8557991 Sawyer RT, et al. Internalin A can mediate phagocytosis of Listeria monocytogenes by mouse macrophage cell lines. J. Leukocyte Biol. 60: 603-610, 1996. PubMed: 8929551 Fleming SD, Campbell PA. Some macrophages kill Listeria monocytogenes while others do not. Immunol. Rev. 158: 69-77, 1997. PubMed: 9314075 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |