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| 产品名称： | GS-109-V-34 |
|---|---|
| 商品货号： | TS212089 |
| Organism： | Homo sapiens, human |
| Product Format： | frozen |
| Morphology： | fibroblast |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | Adenoma |
| Age： | 6 years |
| Gender： | female |
| Ethnicity： | Caucasian |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | This cell line has the normal female karyotype, 46,XX. The modal chromosome number was 46, occurring in 84% of cells, and the rate of polyploidy was 2.8%.The X chromosome was paired. There were no consistent aberrations in all analyzed cells. |
| Clinical Data： | The tissue donor suffered from Gardners syndrome, a form of Familial adenomatous polyposis (FAP). female Caucasian 6 years |
| Tumorigenic： | No |
| Effects： | No, The cells were not tumorigenic in immunosuppressed mice, but did form colonies in semisolid medium. |
| Comments： | The tissue donor suffered from Gardners syndrome, a form of Familial adenomatous polyposis (FAP). It is an autosomal dominant condition with predisposition to carcinoma and multiple polyps of the colon. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
|
| Subculturing： | Protocol:
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended Medium Renewal: 2 to 3 times per week |
| Cryopreservation： | Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Temperature: 37.0°C |
| Name of Depositor： | EJ Gardner |
| Deposited As： | Homo sapiens |