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| 产品名称： | GSML |
|---|---|
| 商品货号： | TS212091 |
| Organism： | Saimiri sciureus sciureus, monkey, Guyanese squirrel |
| Tissue： | peripheral blood, mononuclear cells |
| Cell Type： | B lymphoblast; immortalized with Epstein-Barr virus (EBV)Epstein-Barr virus (EBV) transforme |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 2 Cells contain Epstein Barr viral DNA sequences
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Gender： | male |
| Applications： | GSML is a B lymphocyte cell line established in 2000 by transformation of mononuclear cells from male squirrel monkeys with Epstein-Barr virus derived from B95-8 cells (ATCC CRL-1612). The cells exhibit elevated expression of the FK506-binding immunophilin FKBP51. Chromosome analysis of GSML cells confirm that they are from Guyanese squirrel monkey. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | GSML is a B lymphocyte cell line established in 2000 by transformation of mononuclear cells from male squirrel monkeys with Epstein-Barr virus derived from B95-8 cells (ATCC CRL-1612). |
| Clinical Data： | GSML is a B lymphocyte cell line established in 2000 by transformation of mononuclear cells from male squirrel monkeys with Epstein-Barr virus derived from B95-8 cells (ATCC CRL-1612). male |
| Comments： | GSML is a B lymphocyte cell line established in 2000 by transformation of mononuclear cells from male squirrel monkeys with Epstein-Barr virus derived from B95-8 cells (ATCC CRL-1612). The cells exhibit elevated expression of the FK506-binding immunophilin FKBP51. FKBP51 is the common cause of glucocorticoid resistance. Chromosome analysis of GSML cells confirm that they are from Guyanese squirrel monkey. The Guyanese squirrel monkey and the Bolivian squirrel monkey differ in response to pathologic and pharmacologic stimuli. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
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| Subculturing： | Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 3 X 10 exp5 viable cells/ml. Maintain cell density between 3 X 10 exp5 and 3 X 10 exp6 viable cells/ml. Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density) |
| Cryopreservation： | Freeze medium: Complete growth medium 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Temperature: 37.0°C |
| Population Doubling Time： | 24 hrs |
| Name of Depositor： | JG Scammell |
| Deposited As： | Saimiri sciureus sciureus |
| Year of Origin： | 2000 |
| References： | Scammell JG, et al. The origin of four squirrel monkey cell lines established by karyotype analysis. Cytogenet. Cell Genet. 93: 263-264, 2001. PubMed: 11528123 Scammell JG, et al. Overexpression of the FK506-binding immunophilin FKBP51 is the common cause of glucocorticoid resistance in three New World primates. Gen. Comp. Endocrinol. 124: 152-165, 2001. PubMed: 11703081 |