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| 产品名称： | H1HeLa |
|---|---|
| 商品货号： | TS212095 |
| Organism： | Homo sapiens, human |
| Tissue： | cervix |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 2 xa0Cells contain human papilloma virus (HPV-18)
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | adenocarcinoma |
| Age： | 31 years |
| Gender： | female |
| Ethnicity： | Black |
| Applications： | The H-Hela cell line was established by V. Hamparian from the HeLa cell line isolated by Gey, et al. This subline is susceptible to rhinoviruses and is useful for passaging and titrating rhinoviruses. |
| Derivation： | The H-Hela cell line was established by V. Hamparian from the HeLa cell line isolated by Gey, et al. (see ATCC CCL-2). |
| Clinical Data： | 31 years Black female |
| HeLa Markers： | Y |
| Genes Expressed： | keratin |
| Cellular Products： | keratin |
| Comments： | The H-Hela cell line was established by V. Hamparian from the HeLa cell line isolated by Gey, et al. (see ATCC CCL-2). This subline is susceptible to rhinoviruses and is useful for passaging and titrating rhinoviruses. The line is usually carried as monolayer cultures in Leibovitzs L-15 medium, but can be readily adapted to other media and to suspension culture (in media without Ca++). The H-HeLa line was cured of a mycoplasma contamination by W.M. Lee in Rueckerts laboratory and the cured line was given the designation H1-HeLa. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Leibovitzs L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. (Note: The L-15 medium formulation was devised for use in a free gas exchange with atmospheric air. A CO2 and air mixture is detrimental to cells when using this medium for cultivation) |
| Subculturing： | Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:10 is recommended Medium Renewal: 2 to 3 times per week Remove the medium and add fresh 0.25% trypsin, 0.03% EDTA solution, rinse and remove trypsin. Allow the culture to sit for 5 minutes at room temperature. Add fresh culture medium, aspirate and dispense into new flasks. |
| Culture Conditions： | Atmosphere: air, 100% Temperature: 37°C |
| STR Profile： | Amelogenin: X CSF1PO: 9,10 D13S317: 12,13.3 D16S539: 9,10 D5S818: 11,12 D7S820: 8,12 THO1: 7 TPOX: 8,12 vWA: 16,18 |
| Isoenzymes： | G6PD, A |
| Name of Depositor： | RR Rueckert |
| Deposited As： | Homo sapiens |
| References： | Lee WM Ph.D. thesis, Univ. Wisconsin, 1991 Medappa KC, et al. On the structure of rhinovirus 1A. Virology 44: 259-270, 1971. PubMed: 4327717 |