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微信小章| 产品名称： | H2.8 |
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| 商品货号： | TS212096 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Tissue： | spleen |
| Cell Type： | hybridoma:lymphoblast B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age： | adult |
| Strain： | BALB/c |
| Applications： | ELISA detection zona pellucida glycoprotein 2 ZP2, zona pellucida protein A Western Blot zona pellucida glycoprotein 2 ZP2, zona pellucida protein A detection of zona pellucida glycoprotein 2 ZP2, zona pellucida protein A |
| Storage Conditions： | liquid nitrogen vapor phase |
| Genes Expressed： | zona pellucida glycoprotein 2 |
| Complete Growth Medium： | Dulbeccos modified Eagles medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose supplemented with 10 mM HEPES, 0.15 mg/ml oxaloacetate, 0.05 mg/ml pyruvate, 0.0082 mg/ml bovine insulin and 0.05 mM 2-mercaptoethanol, 80%; fetal bovine serum, 20%
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| Subculturing： | Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation of the suspension with subsequent resuspension in fresh medium at 2 x 105 viable cells/mL. Add medium as the cell density increases. Maintain cultures at cell concentrations between 1 x 105 and 1 x 106 viable cells/mL. Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density) |
| Cryopreservation： | Complete growth medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C |
| Isotype： | IgG1 kappa |
| Name of Depositor： | J Dean |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| References： | Animal were immunized with bacterially expressed, Hi2+-agarose purified 6-histidine tagged recombinant (amino acids 39-642) human ZP2 (zona pellucida glycoprotein 2) Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online. |