微信小陈
微信小章| 产品名称： | GP+E-86 |
|---|---|
| 商品货号： | TS212099 |
| Organism： | Mus musculus, mouse |
| Tissue： | embryo |
| Cell Type： | fibroblast |
| Morphology： | fibroblast |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age： | embryo |
| Strain： | NIH/Swiss |
| Applications： | This line is capable of packaging nucleic acids containing a psi packaging sequence into recombinant ecotropic retrovirus genomes.xa0
It can be used to produce retroviral vectors for delivery of foreign genes into susceptible eukaryotic cells. This cell line is a suitable transfection host.
|
| Storage Conditions： | liquid nitrogen vapor phase |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Derivation： | The line was established by electroporation of two plasmids into NIH 3T3 cells.xa0One plasmid contained the gag and pol regions of Moloney murine leukemia virus (Mo-MuLV), the other contained the env region of Mo-MuLV. |
| Complete Growth Medium： | Dulbeccos modified Eagles medium with 0.015 mg/ml hypoxanthine, 0.25 mg/ml xanthine, and 0.025 mg/ml mycophenolic acid; 90% calf serum, 10%.
|
| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: 1:5 to 1:10 Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a Manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994. |
| Cryopreservation： | Culture medium 95%; DMSO, 5%.xa0Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C |
| Name of Depositor： | Trustees of Columbia University |
| Deposited As： | Mus musculus |
| U.S. Patent Number： | |
| References： | Bank A, et al. Retroviral packaging cell lines and process of using same. US Patent 5,278,056 dated Jan 11 1994 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online. |