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| 产品名称： | GMMe EPI |
|---|---|
| 商品货号： | TS212107 |
| Organism： | Mustela vison, mink |
| Tissue： | uterus; endometrium |
| Cell Type： | Epithelial,fibroblast |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 2 Cells contain SV40 viral DNA sequences
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | Endometrial |
| Age： | adult |
| Gender： | female |
| Applications： | The GMMe (TS212107) and GMMs (ATCC CRL-2675) cell lines were established by stable transfection of endometrial tissue using a plasmid vector, pBAPSV40TtsA58, encoding the SV40 large T antigen driven by the human beta-actin promoter. The cuboidal GMMe cells exhibit epithelial characteristics and the fibroblast GMMs cells exhibit characteristics consistent with a stromal origin. The epithelial cells are strongly positive for cytokeratin and weakly positive for vimentin whereas the fibroblast cells are positive for vimentin and negative for cytokeratin. Both lines are negative for desmin and positive for alkaline phosphatase. These cell lines are used in coculture with mink embryos in obligate diapause to enhance the length and frequency of embryo survival in vitro. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | The GMMe (TS212107) and GMMs (ATCC CRL-2675) cell lines were established by stable transfection of endometrial tissue using a plasmid vector, pBAPSV40TtsA58, encoding the SV40 large T antigen driven by the human beta-actin promoter. |
| Clinical Data： | female |
| Genes Expressed： | alkaline phosphatase,cytokeratin,The epithelial cells are strongly positive for cytokeratin and weakly positive for vimentin whereas the fibroblast cells are positive for vimentin and negative for cytokeratin. |
| Cellular Products： | alkaline phosphatase Ref   Moreau GM, et al. Development of immortalized endometrial epithelial and stromal cell lines from the mink (Mustela vison) uterus and their effects on the survival in vitro of mink blastocysts in obligate diapause. Biol. Reprod. 53: 511-518, 1995. PubMed: 7578673cytokeratin Ref Moreau GM, et al. Development of immortalized endometrial epithelial and stromal cell lines from the mink (Mustela vison) uterus and their effects on the survival in vitro of mink blastocysts in obligate diapause. Biol. Reprod. 53: 511-518, 1995. PubMed: 7578673 |
| Comments： | The GMMe (TS212107) and GMMs (ATCC CRL-2675) cell lines were established by stable transfection of endometrial tissue using a plasmid vector, pBAPSV40TtsA58, encoding the SV40 large T antigen driven by the human beta-actin promoter. The cells were cotransfected with a second plasmid vector, pSV2neo, to confer neomycin resistance. Transfected cells were selected in medium containing G418. The cuboidal GMMe cells exhibit epithelial characteristics and the fibroblast GMMs cells exhibit characteristics consistent with a stromal origin. The epithelial cells are strongly positive for cytokeratin and weakly positive for vimentin whereas the fibroblast cells are positive for vimentin and negative for cytokeratin. Both lines are negative for desmin and positive for alkaline phosphatase. These cell lines are used in coculture with mink embryos in obligate diapause to enhance the length and frequency of embryo survival in vitro. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated DMEM:F12 Medium Catalog No. 30-2006. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing： | Protocol:
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended Medium Renewal: Every 2 to 3 days |
| Cryopreservation： | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C |
| Name of Depositor： | BD Murphy |
| Deposited As： | mink |
| Year of Origin： | June 13, 1994 |
| References： | Moreau GM, et al. Development of immortalized endometrial epithelial and stromal cell lines from the mink (Mustela vison) uterus and their effects on the survival in vitro of mink blastocysts in obligate diapause. Biol. Reprod. 53: 511-518, 1995. PubMed: 7578673 |