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| 产品名称： | GDM-1 |
|---|---|
| 商品货号： | TS212108 |
| Organism： | Homo sapiens, human |
| Tissue： | peripheral blood |
| Cell Type： | monoblast |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | myelomonoblastic leukemia |
| Age： | 66 - 67 years |
| Gender： | female |
| Ethnicity： | White |
| Applications： | This cell line is a model system for studying myelomonocytic disorders and leukemias. |
| Storage Conditions： | liquid nitrogen vapor temperature |
| Derivation： | The GDM-1 cell line was established in 1980 from the peripheral blood of a patient with a Philadelphia chromosome negative myeloproliferative disorder, after transformation to acute myelomonoblastic leukemia. |
| Clinical Data： | 66 - 67 years
female
White |
| Antigen Expression： | Ia +; myeloid leukemia antigen (M-1) Ref   Ben-Bassat H, et al. Establishment and characterization of a new permanent cell line (GDM-1) from a patient with myelomonoblastic leukemia. Leuk. Res. 6: 743-752, 1982. PubMed: 6296552 |
| Receptor Expression： | complement (C3) Ref Ben-Bassat H, et al. Establishment and characterization of a new permanent cell line (GDM-1) from a patient with myelomonoblastic leukemia. Leuk. Res. 6: 743-752, 1982. PubMed: 6296552Fc Ref Ben-Bassat H, et al. Establishment and characterization of a new permanent cell line (GDM-1) from a patient with myelomonoblastic leukemia. Leuk. Res. 6: 743-752, 1982. PubMed: 6296552 |
| Genes Expressed： | lysozyme
Ref
Ben-Bassat H, et al. Establishment and characterization of a new permanent cell line (GDM-1) from a patient with myelomonoblastic leukemia. Leuk. Res. 6: 743-752, 1982. PubMed: 6296552myeloperoxidase
Ref
Ben-Bassat H, et al. Establishment and characterization of a new permanent cell line (GDM-1) from a patient with myelomonoblastic leukemia. Leuk. Res. 6: 743-752, 1982. PubMed: 6296552 |
| Cellular Products： | lysozyme
Ref
Ben-Bassat H, et al. Establishment and characterization of a new permanent cell line (GDM-1) from a patient with myelomonoblastic leukemia. Leuk. Res. 6: 743-752, 1982. PubMed: 6296552myeloperoxidase
Ref Ben-Bassat H, et al. Establishment and characterization of a new permanent cell line (GDM-1) from a patient with myelomonoblastic leukemia. Leuk. Res. 6: 743-752, 1982. PubMed: 6296552 |
| Tumorigenic： | No |
| Effects： | No, did not form colonies in soft agar |
| Comments： | The cells lack B- and T-cell surface markers including T-associated antigens, E-rosetting capacity, surface and intracytoplasmic immunoglobulins. They are non-specific esterase positive. The cells are phagocytic for latex beads and iron particles. Exposure to phorbol 12-myristate 13-acetate (TPA) increases the phagocytic activity. TPA also induces macrophage-like differentiation. The cells are negative for Epstein-Barr virus nuclear antigen (EBNA-). |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
|
| Subculturing： | Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium at 3 - 5 x 10 5 viable cells/mL. Maintain cultures at cell concentrations between 3 x 10 5 and 3 x 106 viable cells/mL. Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density). |
| Cryopreservation： | Freeze medium: Complete growth medium 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor temperature |
| Culture Conditions： | Temperature: 37°C
|
| Population Doubling Time： | 24 to 36 hrs |
| Name of Depositor： | H Ben-Bassat |
| Deposited As： | human |
| Year of Origin： | 1980 |
| References： | Ben-Bassat H, et al. Establishment and characterization of a new permanent cell line (GDM-1) from a patient with myelomonoblastic leukemia. Leuk. Res. 6: 743-752, 1982. PubMed: 6296552 |