微信小陈
微信小章
产品简介
购买须知
| 产品名称： | Genox 3.53 |
|---|---|
| 商品货号： | TS212111 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | The antibody reacts with the DQw1 (DC10) determinant of the HLA D region. Tested and found negative for ectromelia virus (mousepox). |
| Derivation： | Spleen cells were fused with NS-1 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against DQw1 (DC1, HLA DR) |
| Cellular Products： | immunoglobulin; monoclonal antibody; against DQw1 (DC1, HLA DR) |
| Comments： | Animals were immunized with glycoproteins from the Bristol 8 human B lymphoblastoid cell line. Spleen cells were fused with NS-1 myeloma cells. The antibody reacts with the DQw1 (DC10) determinant of the HLA D region. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | Culture Medium: Dulbeccos modified Eagles medium with 4.5 g/L glucose, 90%; fetal bovine serum, 10%. |
| Subculturing： | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Isotype： | IgG1 |
| Name of Depositor： | P Parham |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| References： | Brodsky FM, et al. Monoclonal antibodies to HLA -- DRw determinants. Tissue Antigens 16: 30-48, 1980. PubMed: 6162228 |