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| 产品名称： | GL7 |
|---|---|
| 商品货号： | TS212117 |
| Organism： | Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | The antibody reacts with a 35000 dalton antigen present on subsets of activated mouse B cells and activated CD4 + and CD 8 + mouse T cells. |
| Derivation： | Spleen cells were fused with Sp2/0-Ag14 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against mouse lymphocytes |
| Cellular Products： | immunoglobulin; monoclonal antibody; against mouse lymphocytes |
| Comments： | Animals were immunized with LPS activated DBA/2J mouse B cells. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody reacts with a 35000 dalton antigen present on subsets of activated mouse B cells and activated CD4 + and CD 8 + mouse T cells. The GL7 antigen is expressed on up to 70% of activated B cells and on high proportions of ConA activated CD4 + and CD8 + T cells. Unstimulated B and T cells are infrequently GL7 positive. The antigen appears to be a lymphocyte activation antigen that is different from those previously identified. |
| Complete Growth Medium： | Culture Medium: RPMI 1640 medium with 2 mM L-glutamine, 1 mM sodium pyruvate, 1% nonessential amino acids and 50 ?M 2-mercaptoethanol, 90%; fetal bovine serum, 10%. |
| Subculturing： | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Cryopreservation： | Culture medium, 95%; DMSO, 5% |
| Culture Conditions： | Temperature: 37.0°C |
| Isotype： | IgM |
| Name of Depositor： | G Lazlo, KS Hathcock |
| Deposited As： | rat (B cell); mouse (myeloma) |
| References： | Laszlo G, et al. Characterization of a novel cell-surface molecule expressed on subpopulations of activated T and B cells. J. Immunol. 150: 5252-5262, 1993. PubMed: 8515058 |