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| 产品名称： | GAP A3 |
|---|---|
| 商品货号： | TS212120 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension, suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | Tested and found negative for ectromelia virus (mousepox). |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | Spleen cells were fused with Sp2/0-Ag14 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against HLA A3 |
| Cellular Products： | immunoglobulin; monoclonal antibody; against HLA A3 |
| Comments： | Animals were immunized with human peripheral blood lymphocytes. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody does not react with HLA A11. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell culture grade water. To make the complete growth medium, add the following components to the base medium: |
| Subculturing： | Protocol: Cultures can be maintained by addition or replacement of medium. Establish new cultures at 1 X 10(5) viable cells/ml and maintain between 2 X 10(5) and 1 X 10(6) cells/ml. Medium Renewal: Every 2 to 3 days |
| Cryopreservation： | Freeze medium: culture medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Isotype： | IgG2a; kappa light chain |
| Name of Depositor： | AE Berger |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| References： | . . Fed. Proc. 42: 1371, 1983. Dieckmann CL, et al. Monoclonal antibody to HLA-A3. Hybridoma 1: 87-90, 1982. PubMed: 6208124 |