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微信小章| 产品名称： | GCT Giant Cell Tumor |
|---|---|
| 商品货号： | TS212127 |
| Organism： | Homo sapiens, human |
| Tissue： | derived from metastatic site: lung |
| Product Format： | frozen |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | fibrous histiocytoma |
| Age： | 29 years |
| Gender： | male |
| Applications： | The line produces CSA for human granulocyte precursors and EEA for erythroid precursor.xa0Medium conditioned by this line can be used as a source of prostaglandin E and plasminogen activator. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Clinical Data： | male
29 years |
| Genes Expressed： | colony stimulating activity (CSA); erythroid enhancing activity (EEA); prostaglandin E; plasminogen activator |
| Cellular Products： | colony stimulating activity (CSA); erythroid enhancing activity (EEA); prostaglandin E; plasminogen activator |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated McCoys 5a Medium Modified, Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: 1:2 to 1:4 Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 13 in Culture Of Animal Cells: A Manual Of Basic Technique by R. Ian Freshney, 5th edition, published by Wiley-Liss, N.Y., 2005. |
| Cryopreservation： | Complete growth medium described above supplemented with 5% (v/v) DMSO. Cell culture tested DMSO is available as ATCC® Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| STR Profile： | Amelogenin: X CSF1PO: 12 D13S317: 11,12 D16S539: 9 D5S818: 13,15 D7S820: 11,12 THO1: 8,9.3 TPOX: 8,9 vWA: 16,18 |
| Name of Depositor： | J Brennan |
| Deposited As： | Homo sapiens |
| References： | Di Persio JF, et al. Human cell lines that elaborate colony-stimulating activity for the marrow cells of man and other species. Blood 51: 507-519, 1978. PubMed: 304748 DiPersio JF, et al. The fractionation, characterization, and subcellular localization of colony-stimulating activities released by the human monocyte-like cell line, GCT. Blood 56: 717-727, 1980. PubMed: 6968234 Abboud CN, et al. Hydrophobic adsorption chromatography of colony-stimulating activities and erythroid-enhancing activity from the human monocyte-like cell line, GCT. Blood 58: 1148-1154, 1981. PubMed: 6975643 Lichtman MA, Brennan JK. Obtaining human cell lines that elaborate colony stimulating activity for marrow cells of man and other species and methods of preparing same. US Patent 4,135,975 dated Jan 23 1979 Abboud CN, et al. Erythropoietic enhancing activity (EEA) secreted by the human cell line, GCT. J. Supramol. Struct. 13: 199-209, 1980. PubMed: 6972469 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |