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微信小章| 产品名称： | G-7 |
|---|---|
| 商品货号： | TS212128 |
| Organism： | Mus musculus, mouse |
| Tissue： | skeletal muscle |
| Cell Type： | myoblast myoblast |
| Product Format： | frozen |
| Morphology： | myoblast |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age： | fetus fetus |
| Strain： | Swiss |
| Applications： | They are slightly responsive to acetylcholine. To avoid this, the cells should be subcultured before they become confluent, and they should be recloned periodically with selection for myoblastic clones. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | modal number = 40 |
| Tumorigenic： | Yes |
| Effects： | Yes, |
| Comments： | When confluent, the cells fuse to form multinucleated myotubes. They are slightly responsive to acetylcholine. The myoblastic component of the population will rapidly become depleted if the cells are allowed to become confluent, since more extensive fusion will take place. To avoid this, the cells should be subcultured before they become confluent, and they should be recloned periodically with selection for myoblastic clones. |
| Complete Growth Medium： | Dulbeccos modified Eagles medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose, 80%; horse serum, 10%; fetal bovine serum, 10%
Dulbeccos modified Eagles medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose, 80%; horse serum, 10%; fetal bovine serum, 10% |
| Subculturing： | The cells should be subcultured before they become confluent. Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: 1:2 to 1:4 |
| Cryopreservation： | Complete growth medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| Population Doubling Time： | 17 to 19 hrs |
| Name of Depositor： | J Peacock |
| Deposited As： | Mus musculus |
| References： | Christian CN, et al. Synapse formation between two clonal cell lines. Science 196: 995-998, 1977. PubMed: 193191 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |