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| 产品名称： | G-361 |
|---|---|
| 商品货号： | TS212133 |
| Organism： | Homo sapiens, human |
| Tissue： | skin |
| Product Format： | frozen |
| Morphology： | epithelial |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | malignant melanoma |
| Age： | 31 years adult |
| Gender： | male |
| Ethnicity： | Caucasian |
| Clinical Data： | 31 years adult Caucasian male |
| Genes Expressed： | melanin |
| Cellular Products： | melanin |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated McCoys 5a Medium Modified, Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing： | Remove medium, add fresh 0.25% trypsin - 0.53 mM EDTA for several minutes, remove trypsin and let the culture sit at 37°C for 10 to 15 minutes. Add fresh medium, aspirate and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: 24 hours after seeding from a thawed ampule, then 2 to 3 times per week |
| Culture Conditions： | Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C |
| STR Profile： | Amelogenin: X CSF1PO: 10 D13S317: 11 D16S539: 15 D5S818: 11 D7S820: 8,11 THO1: 7,9 TPOX: 8,10 vWA: 15,17 |
| Isoenzymes： | G6PD, B |
| Name of Depositor： | PT Peebles |
| Deposited As： | Homo sapiens |
| References： | Peebles P, et al. Isolation of four unusual pediatric solid tumor cell lines. Pediatr. Res. 12: 485, 1978. |