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微信小章| 产品名称： | G-8 |
|---|---|
| 商品货号： | TS212135 |
| Organism： | Mus musculus, mouse |
| Tissue： | skeletal muscle |
| Cell Type： | myoblast myoblast |
| Product Format： | frozen |
| Morphology： | myoblast |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age： | fetus fetus |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | modal number = 66 |
| Receptor Expression： | acetylcholine (The cells are highly responsive to acetylcholine.) |
| Genes Expressed： | myosin |
| Cellular Products： | myosin |
| Tumorigenic： | No |
| Effects： | No, in immunosuppressed mice Yes, semi-solid media |
| Complete Growth Medium： | Dulbeccos modified Eagles medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose, 80%; horse serum, 10%; fetal bovine serum, 10%
Dulbeccos modified Eagles medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose, 80%; horse serum, 10%; fetal bovine serum, 10% |
| Subculturing： | The cells should be subcultured before they become confluent. Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: 1:2 to 1:4 |
| Cryopreservation： | Complete growth medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| Population Doubling Time： | 25 to 27 hrs |
| Name of Depositor： | J Peacock |
| Deposited As： | Mus musculus |
| References： | Christian CN, et al. Synapse formation between two clonal cell lines. Science 196: 995-998, 1977. PubMed: 193191 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |