Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

This line was originally thought to be derived from normal amnion, but was subsequently found, based on isoenzyme analysis, HeLa marker chromosomes, and DNA fingerprinting, to have been established via HeLa cell contamination.

The cells are positive for keratin by immunoperoxidase staining.

NOTE: Cells of this line contain HeLa marker chromosomes, and were derived via HeLa contamination

This line was originally thought to be derived from normal amnion, but was subsequently found, based on isoenzyme analysis, HeLa marker chromosomes, and DNA fingerprinting, to have been established via HeLa cell contamination.

female

keratin,The cells are positive for keratin by immunoperoxidase staining.

keratin

This line was originally thought to be derived from normal amnion, but was subsequently found, based on isoenzyme analysis, HeLa marker chromosomes, and DNA fingerprinting, to have been established via HeLa cell contamination.

The cells are positive for keratin by immunoperoxidase staining.

NOTE: Cells of this line contain HeLa marker chromosomes, and were derived via HeLa contamination

Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:10 is recommended

Medium Renewal: 2 to 3 times per week

Protocol: Volumes used in this protocol are for 75 sq cm flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.

• Remove and discard culture medium.
• Briefly rinse the cell layer with Ca++/Mg++ free Dulbeccos phosphate-buffered saline (D-PBS) or 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
• Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
  Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.
• Add 2.0 to 3.0 ml of complete growth medium and aspirate cells by gently pipetting
• Resuspend the cell pellet in fresh growth medium. Add appropriate aliquots of the cell suspension to new culture vessels.
• Incubate cultures at 37C.

Temperature: 37.0°C

Amelogenin: X

CSF1PO: 9,10

D13S317: 12,13.3

D16S539: 9,10

D5S818: 11,12

D7S820: 8,12

THO1: 7

TPOX: 8,12

vWA: 16,18

G6PD, A