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微信小章| 产品名称： | FHs 74 Int |
|---|---|
| 商品货号： | TS212153 |
| Organism： | Homo sapiens, human |
| Tissue： | small intestine |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | normal |
| Age： | 3 to 4 months gestation |
| Gender： | female |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | normal female; diploid |
| Images： |  |
| Clinical Data： | female |
| Receptor Expression： | epidermal growth factor (EGF), expressed |
| Tumorigenic： | No |
| Effects： | No, in immunosuppressed mice Yes, in semisolid medium |
| Comments： | PAS, negative. The cells are negative for keratin by immunoperoxidase staining. |
| Complete Growth Medium： | Hybri-Care Medium ATCC 46-X supplemented with 30 ng/ml epidermal growth factor (EGF), 90%; fetal bovine serum, 10% (Do not filter the medium after addition of EGF) |
| Subculturing： | Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:4 is recommended Medium Renewal: Every 2 to 3 days |
| Cryopreservation： | Freeze Medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage Temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Atmosphere: Air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C |
| STR Profile： | Amelogenin: X CSF1PO: 10,11 D13S317: 11,12 D16S539: 11,12 D5S818: 11,12 D7S820: 9,10 THO1: 7,9.3 TPOX: 8 vWA: 16,18 |
| Isoenzymes： | AK-1, 1 ES-D, 1-2 G6PD, B GLO-I, 1 Me-2, 1-2 PGM1, 1 PGM3, 1 |
| Name of Depositor： | R Owens |
| Deposited As： | Homo sapiens |
| References： | Owens RB, et al. Epithelial cell cultures from normal and cancerous human tissues. J. Natl. Cancer Inst. 56: 843-849, 1976. PubMed: 176412 Smith HS. In vitro properties of epithelial cell lines established from human carcinomas and nonmalignant tissue. J. Natl. Cancer Inst. 62: 225-230, 1979. PubMed: 283258 Smith HS, et al. Nuclear ultrastructure of epithelial cell lines derived from human carcinomas and nonmalignant tissues. Cancer Res. 39: 332-334, 1979. PubMed: 761205 |