FHC

货号	TS212162
中文名称	null
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产品简介

购买须知

产品名称：

FHC

商品货号：

TS212162

Organism：

Homo sapiens, human

Tissue：

colon

Cell Type：

Epithelial

Product Format：

frozen

Morphology：

epithelial

Culture Properties：

adherent

Biosafety Level：

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease：

normal

Age：

13 weeks gestation

Storage Conditions：

liquid nitrogen vapor phase

Images：

Comments：

When using a non-humidified incubator, gassing of cultures with 5% CO2 and plug-seal caps should be used to prevent evaporation of liquid in media and unintentional concentrating of media additives.

Although FHC cells exhibit epithelial morphology, cytoplasmic keratins were not detected.

Complete Growth Medium：

The base medium for this cell line is DMEM:F12 Medium (ATCC 30-2006).To make the complete growth medium, add the following components to the base medium:

extra 10 mM HEPES (for a final conc. of 25 mM)
10 ng/ml cholera toxin
0.005 mg/ml insulin
0.005 mg/ml transferrin
100 ng/ml hydrocortisone
20 ng/mL human recombinant EGF (Thermo Fisher PHG0311)
Fetal Bovine Serum, 10% final conc (ATCC 30-2020)

The base medium for this cell line is ATCC-formulated DMEM:F12 Medium Catalog No. 30-2006.To make the complete growth medium, add the following components to the base medium: extra 10 mM HEPES (for a final conc. of 25 mM) 10 ng/ml cholera toxin 0.005 mg/ml insulin 0.005 mg/ml transferrin 100 ng/ml hydrocortisone fetal bovine serum 10%(final conc.
Subculturing：	The base medium for this cell line is ATCC-formulated DMEM:F12 Medium Catalog No. 30-2006.To make the complete growth medium, add the following components to the base medium: extra 10 mM HEPES (for a final conc. of 25 mM) 10 ng/ml cholera toxin 0.005 mg/ml insulin 0.005 mg/ml transferrin 100 ng/ml hydrocortisone fetal bovine serum 10%(final conc.
Cryopreservation：	Volumes are given for a 75 cm² flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. To remove trypsin-EDTA solution, transfer cell suspension to centrifuge tube and spin at approximately 125 xg for 5 to 10 minutes. Discard supernatant and resuspend cells in fresh growth medium. Add appropriate aliquots of cell suspension to new culture vessels. An inoculum of 4 to 6 X 10³ viable cell/cm² is recommended. Incubate cultures at 37°C. Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended every 10 to 15 days Medium Renewal: Every 3 to 4 days.
Culture Conditions：	Freeze Medium: Complete growth medium supplemented with an extra 50% FBS and 10% (v/v) DMSO Storage Temperature: Liquid nitrogen vapor phase
STR Profile：	Temperature: 37°C Atmosphere: Air, 95%; carbon dioxide (CO₂), 5%
Name of Depositor：	Amelogenin: X,Y CSF1PO: 11,12 D13S317: 12,13 D16S539: 9,11 D5S818: 12,13 D7S820: 8,12 THO1: 6,9.3 TPOX: 8,11 vWA: 16
Deposited As：	DP Chopra
References：	Homo sapiens

序号	文章标题	操作
1	ATCC细胞运输和处理	查看详情
2	其他海外品牌代理	查看详情
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4	购买须知	查看详情
5	订购流程	查看详情

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