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购买须知
| 产品名称： | FHs 173We |
|---|---|
| 商品货号： | TS212167 |
| Organism： | Homo sapiens, human |
| Tissue： | fetus, whole |
| Product Format： | frozen |
| Morphology： | fibroblast |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | normal |
| Age： | fetus |
| Gender： | male |
| Ethnicity： | Black |
| Storage Conditions： | liquid nitrogen vapor phase |
| Clinical Data： | male Black fetus |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing： | Protocol: Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended Medium Renewal: 2 to 3 times per week |
| Cryopreservation： | Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Temperature: 37.0°C |
| STR Profile： | Amelogenin: X,Y CSF1PO: 10,11 D13S317: 12 D16S539: 9,12 D5S818: 10,12 D7S820: 8,11 THO1: 7,8 TPOX: 8,9 vWA: 15,16 |
| Isoenzymes： | AK-1, 1 ES-D, 1-2 G6PD, B GLO-I, 2 PGM1, 1 PGM3, 1-2 |
| Name of Depositor： | RB Owens |
| Deposited As： | Homo sapiens |