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微信小章| 产品名称： | FBHE |
|---|---|
| 商品货号： | TS212171 |
| Organism： | Bos taurus, cow |
| Tissue： | heart; vascular endothelium |
| Cell Type： | endothelial |
| Product Format： | frozen |
| Morphology： | endothelial |
| Culture Properties： | adherent |
| Biosafety Level： | 2 xa0Cells contain bovine vral diarrhea virus (BVDV)
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | normal |
| Age： | fetus |
| Applications： | The line is positive for bovine viral diarrhea virus (BVD). |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | The FBHE cell line absolutely requires fibroblast growth factor (FGF) for survival, which can be supplied in the form of purified FGF or Bovine Pituitary Extract (BPE). |
| Comments： | The FBHE cell line absolutely requires fibroblast growth factor (FGF) for survival, which can be supplied in the form of purified FGF or Bovine Pituitary Extract (BPE). The line is positive for bovine viral diarrhea virus (BVD). |
| Complete Growth Medium： | Dulbeccos modified Eagles medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose (ATCC® No. 30-2002) and supplemented with 50 μg/mLxa0bovine pituitary extract, 90%; bovine calf serum (ATCC® No. 30-2030), 10%. |
| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Note: Subculture every 5 to 7 days, before 90% confluency.
Subcultivation Ratio: 1:2 to 1:5 Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 13 in Culture of Animal Cells, a Manual of Basic Technique by R. Ian Freshney,xa05th edition, published by Wiley-Liss, N.Y., 2005. |
| Cryopreservation： | Complete growth medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| Name of Depositor： | DJ Gospodarowicz |
| Deposited As： | Bos taurus |
| References： | Bolin SR, et al. Survey of cell lines in the American Type Culture Collection for bovine viral diarrhea virus. J. Virol. Methods 48: 211-221, 1994. PubMed: 7989438 Gospodarowicz D, et al. Clonal growth of bovine vascular endothelial cells: fibroblast growth factor as a survival agent. Proc. Natl. Acad. Sci. USA 73: 4120-4124, 1976. PubMed: 1069301 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |