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微信小章| 产品名称： | F9 |
|---|---|
| 商品货号： | TS212182 |
| Organism： | Mus musculus, mouse |
| Tissue： | testis |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | embryonal carcinoma; testicular teratoma |
| Age： | embryo |
| Strain： | 129 |
| Applications： | This cell line is a suitable transfection host. |
| Genes Expressed： | plasminogen activator; laminin; type IV collagen |
| Cellular Products： | plasminogen activator; laminin; type IV collagen |
| Comments： | F9 cells can be stimulated to differentiate into parietal endoderm in the presence of retinoic acid and dibutyryl cyclic AMP (cAMP). Differentiating cells synthesize plasminogen activator, laminin and type IV collagen. cAMP is active only on cells that have been treated with retinoic acid. The cells maintain three copies of the beta 1 integrin gene. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes. NOTE: culture vessels must be coated with 0.1% gelatin prior to use.
To do so, cover the surface of the vessel with 0.1% gelatin from porcine skin (Sigma G2500 or G1890) in sterile distilled water for 2 hours at 4°C, then wash three times with sterile distilled water. Treated flasks and dishes can be stored at room temperature.
Subcultivation Ratio: A subcultivation ratio of 1:10 is recommended
Medium Renewal: Every 2 to 3 days |
| Culture Conditions： | Temperature: 37°C |
| Name of Depositor： | S Strickland |
| Deposited As： | Mus musculus |
| References： | Strickland S, et al. Hormonal induction of differentiation in teratocarcinoma stem cells: generation of parietal endoderm by retinoic acid and dibutyryl cAMP. Cell 21: 347-355, 1980. PubMed: 6250719 Strickland S, Mahdavi V. The induction of differentiation in teratocarcinoma stem cells by retinoic acid. Cell 15: 393-403, 1978. PubMed: 214238 Stephens LE, et al. Targeted deletion of beta 1 integrins in F9 embryonal carcinoma cells affects morphological differentiation but not tissue-specific gene expression. J. Cell Biol. 123: 1607-1620, 1993. PubMed: 7504677 Berstine EG, et al. Alkaline phosphatase activity in mouse teratoma. Proc. Natl. Acad. Sci. USA 70: 3899-3903, 1973. PubMed: 4521215 Jang SI, et al. Activator protein 1 activity is involved in the regulation of the cell type-specific expression from the proximal promoter of the human profilaggrin gene. J. Biol. Chem. 271: 24105-24114, 1996. PubMed: 8798649 |