微信小陈
微信小章| 产品名称： | FaDu |
|---|---|
| 商品货号： | TS212183 |
| Organism： | Homo sapiens, human |
| Tissue： | pharynx |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | squamous cell carcinoma |
| Age： | 56 years |
| Gender： | male |
| Ethnicity： | Caucasian |
| Applications： | This cell line is a suitable transfection host. |
| Karyotype： | (P16) hypodiploid to hypertriploid with modal number = 64 |
| Derivation： | The FaDu line was established in 1968 from a punch biopsy of an hypopharyngeal tumor removed from a patient. |
| Clinical Data： | 56 years Caucasian male The FaDu line was established in 1968 from a punch biopsy of an hypopharyngeal tumor removed from a patient. |
| Tumorigenic： | Yes |
| Effects： | Yes, in nude mice; forms well differentiated epidermoid carcinoma (grade I) |
| Comments： | The established line was found to contain bundles of tonofilaments in the cell cytoplasm and desmosomal regions were prominent at cell boundaries. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing： | Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week |
| Cryopreservation： | Culture medium, 95%; DMSO, 5% |
| Culture Conditions： | Temperature: 37°C |
| STR Profile： | Amelogenin: None detected
CSF1PO: 12
D13S317: 8, 9
D16S539: 11
D5S818: 12
D7S820: 11, 12
THO1: 8
TPOX: 11
vWA: 15, 17 |
| Isoenzymes： | AK-1, 1 ES-D, 1 G6PD, B GLO-I, 2 Me-2, 2 PGM1, 2 PGM3, 1 |
| Name of Depositor： | SR Rangan |
| Deposited As： | Homo sapiens |
| Year of Origin： | 1968 |
| References： | Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871 Goodfellow M, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 77210034 Rangan SR. A new human cell line (FaDu) from a hypopharyngeal carcinoma. Cancer 29: 117-121, 1972. PubMed: 4332311 Faust JB, Meeker TC. Amplification and expression of the bcl-1 gene in human solid tumor cell lines. Cancer Res. 52: 2460-2463, 1992. PubMed: 1568216 Giard DJ, et al. In vitro cultivation of human tumors: establishment of cell lines derived from a series of solid tumors. J. Natl. Cancer Inst. 51: 1417-1423, 1973. PubMed: 4357758 |