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微信小章| 产品名称： | FAT 7 |
|---|---|
| 商品货号： | TS212185 |
| Organism： | Rattus norvegicus, rat |
| Tissue： | nasal |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | squamous cell carcinoma |
| Age： | adult |
| Gender： | male |
| Applications： | The FAT 7 line was established from a nasal squamous cell carcinoma induced by formaldehyde inhalation in an adult male rat. The cells contain a point mutation in the conserved V region of the p53 gene at codon 271 (CGT --> CAT transversion). |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | The FAT 7 line was established from a nasal squamous cell carcinoma induced by formaldehyde inhalation in an adult male rat. |
| Clinical Data： | The FAT 7 line was established from a nasal squamous cell carcinoma induced by formaldehyde inhalation in an adult male rat. male |
| Receptor Expression： | epidermal growth factor (EGF) |
| Genes Expressed： | transforming growth factor alpha (TGF alpha) |
| Cellular Products： | transforming growth factor alpha (TGF alpha) |
| Tumorigenic： | Yes |
| Effects： | Yes, in female CD-1 nude mice |
| Comments： | The FAT 7 line was established from a nasal squamous cell carcinoma induced by formaldehyde inhalation in an adult male rat. The cells contain a point mutation in the conserved V region of the p53 gene at codon 271 (CGT --> CAT transversion). |
| Complete Growth Medium： | Hams F12K medium with 0.01 mg/ml insulin, 250 ng/ml hydrocortisone and 0.0025 mg/ml transferrin, 90%; fetal bovine serum, 10%
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| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: 1:5 to 1:10 Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 13 in Culture of Animal Cells, a Manual of Basic Technique by R. Ian Freshney,xa05th edition, published by Wiley-Liss, N.Y., 2005. |
| Cryopreservation： | Complete growth medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| Population Doubling Time： | 24 hrs |
| Name of Depositor： | E Bermudez |
| Deposited As： | Rattus sp. |
| References： | Bermudez E, et al. Characterization of cell lines derived from formaldehyde-induced nasal tumors in rats. Mol. Carcinog. 9: 193-199, 1994. PubMed: 8148052 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |