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微信小章| 产品名称： | F19 |
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| 商品货号： | TS212188 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Tissue： | spleen |
| Cell Type： | hybridoma: lymphoblast B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | sarcoma |
| Age： | adult |
| Strain： | (BALB/c x C57BL/6)F1 |
| Applications： | The antibody reacts with a glycoprotein(s) (gp120/95), of relative molecular weight 120000 and 95000 daltons respectively, present on cultured human fibroblasts and most sarcomas.xa0The F19 antigen is not detected on SV40 transformed fibroblasts |
| Storage Conditions： | liquid nitrogen vapor phase |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Derivation： | Animals were immunized with human lung fibroblasts. |
| Comments： | The antibody reacts with a glycoprotein(s) (gp120/95), of relative molecular weight 120000 and 95000 daltons respectively, present on cultured human fibroblasts and most sarcomas. The F19 antigen is not detected on SV40 transformed fibroblasts. During scar formation, the antigen is expressed in the scar but not in the surrounding tissue. The F19 antigen is also often expressed in the normal stroma surrounding the growth of tumors. A culture deposited with the ATCC as HB-8269 in March of 1983 was found to be contaminated with mycoplasma. Progeny were cured by a 21-day treatment with BM Cycline. The cured cell line is available as TS212188. The original patent deposit is available as HB-8269. |
| Complete Growth Medium： | RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate and supplemented with 0.1 mM non-essential amino acids, 90%; fetal bovine serum, 10%
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| Subculturing： | Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 3 x 104 viable cells/mL.xa0Maintain cultures at a cell concentration between 3 x 104 and 3 x 105 cells/mL.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density). |
| Cryopreservation： | Complete growth medium supplemented with 5% (v/v) DMSO. Cell culture tested DMSO is available as ATCC® Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| Isotype： | mouse IgG1 kappa |
| Name of Depositor： | Sloan-Kettering Institute for Cancer Research |
| U.S. Patent Number： | |
| References： | Rettig WJ, et al. Cell-surface glycoproteins of human sarcomas. US Patent 5,059,523 dated Oct 22 1991 Rettig WJ, et al. Differential expression of cell surface antigens and glial fibrillary acidic protein in human astrocytoma subsets. Cancer Res. 46: 6406-6412, 1986. PubMed: 2877731 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |