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| 产品名称： | Ep-16 |
|---|---|
| 商品货号： | TS212200 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | The antibody reacts with an epithelial specific antigen on human foreskin keratinocytes but does not react with mesodermal cells or lymphocytes. Tested and found negative for ectromelia virus (mousepox). |
| Derivation： | Spleen cells were fused with NS-1 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against human epithelium |
| Cellular Products： | immunoglobulin; monoclonal antibody; against human epithelium |
| Comments： | Animals were immunized with a membrane enriched extract of human foreskin keratinocytes. Spleen cells were fused with NS-1 myeloma cells. The antibody reacts with an epithelial specific antigen on human foreskin keratinocytes but does not react with mesodermal cells or lymphocytes. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | Culture Medium: RPMI medium 1640, 90%; fetal bovine serum, 10%. |
| Subculturing： | Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 X 10(5) cells/ml and maintain between 1 X 10(5) and 1 X 10(6) cells/ml. Medium Renewal: Every 2 to 3 days |
| Isotype： | IgM; kappa light chain |
| Name of Depositor： | AW Hamburger |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| References： | Hamburger AW, et al. Isolation and characterization of a monoclonal antibody specific for epithelial cells. Cancer Res. 45: 783-790, 1985. PubMed: 2578308 |