微信小陈
微信小章| 产品名称： | EOC 2 |
|---|---|
| 商品货号： | TS212201 |
| Organism： | Mus musculus, mouse |
| Tissue： | brain |
| Cell Type： | microglia |
| Product Format： | frozen |
| Morphology： | macrophage |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age： | 10 days |
| Gender： | female |
| Strain： | C3H/HeJ |
| Applications： | The cells may be used to characterize the role of brain macrophages. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Images： |  |
| Derivation： | This is an immortalized cell line derived from the brain of an apparently normal 10 day old mouse. Cells were cloned in soft agar in the presence of CSF-1 and expanded on microcarrier beads. Beads were transferred to culture dishes and were subsequently passaged by scraping. |
| Clinical Data： | female
10 days |
| Antigen Expression： | CD11b/CD18 (Mac-1) +, Mac-2 +, Mac-3 +, CD80 (B7-1) +, CD86 (B7.2) +; CD45 +, Ly-6C +, F4/80 +, MHC Class I +, MHC Class II +, CD115 (colony stimulating factor 1 receptor (CSF-1R)) +, FcR + |
| Receptor Expression： | colony stimulating factor 1 (CSF-1R, CD115) |
| Genes Expressed： | CD11b/CD18 (Mac-1) +, Mac-2 +, Mac-3 +, CD80 (B7-1) +, CD86 (B7.2) +; CD45 +, Ly-6C +, F4/80 +, MHC Class I +, MHC Class II +, CD115 (colony stimulating factor 1 receptor (CSF-1R)) +, FcR + |
| Comments： | The cell line is dependent on colony stimulating factor 1 (CSF-1).
The cells exhibit phagocytic activity.
Unlike EOC 13.31 (ATCC CRL-2468) and EOC 20 (ATCC CRL-2469), these cells do not constitutively express high levels of major histocompatibility complex (MHC) class II antigens and expression is not upregulated by recombinant murine interferon-gamma. C3H/HeJ strain is defective in TLR4 (toll-like receptor 4) |
| Complete Growth Medium： | Dulbeccos modified Eagles medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose, 70%; fetal bovine serum, 10%; LADMAC Conditioned Media (produced from the LADMAC cell line (CRL-2420), 20%
|
| Subculturing： |
Subcultivation Ratio: 1:2 to 1:4 Medium Renewal: Every 2 to 3 days |
| Cryopreservation： | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Temperature: 37°C |
| Name of Depositor： | WS Walker |
| Deposited As： | mouse |
| References： | Olivas E, et al. Use of the Pannell-Milstein roller bottle apparatus to produce high concentrations of the CSF-1, the mouse macrophage growth factor. J. Immunol. Methods 182: 73-79, 1995. PubMed: 7769247 Walker WS. Establishment of mononuclear phagocyte cell lines. J. Immunol. Methods 174: 25-31, 1994. PubMed: 8083530 Walker WS, et al. Mouse microglial cell lines differing in constitutive and interferon-gamma-inducible antigen-presenting activities for naive and memory CD4+ and CD8+ T cells. J. Neuroimmunol. 63: 163-174, 1995. PubMed: 8550814 Askew D, Walker WS. Alloantigen presentation to naive CD8+ T cells by mouse microglia: evidence for a distinct phenotype based on expression of surface-associated and soluble costimulatory molecules. Glia 18: 118-128, 1996. PubMed: 8913775 |