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微信小章| 产品名称： | EOMA |
|---|---|
| 商品货号： | TS212202 |
| Organism： | Mus musculus, mouse |
| Tissue： | tumor |
| Cell Type： | endothelial |
| Product Format： | frozen |
| Morphology： | endothelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | hemangioendothelioma |
| Age： | adult |
| Strain： | 129 |
| Storage Conditions： | liquid nitrogen vapor phase |
| Images： |  |
| Derivation： | The EOMA cell line was originally derived in 1980 from a mixed hemangioendothelioma arising in an adult mouse. |
| Antigen Expression： | CD31 + vascular addressin + CD45 (Ly5-T200) + |
| Receptor Expression： | acetylated low density liproprotein Ref   Obeso J, et al. A hemangioendothelioma-derived cell line: its use as a model for the study of endothelial cell biology. Lab. Invest. 63: 259-269, 1990. PubMed: 2166185 |
| Genes Expressed： | angiotensin converting enzyme (ACE)
thrombospondin
cathepsin L
endostatin
interleukin-6 (interleukin 6, IL-6)
|
| Cellular Products： | angiotensin converting enzyme (ACE)
thrombospondin
cathepsin L
endostatin
interleukin-6 (interleukin 6, IL-6) |
| Tumorigenic： | Yes |
| Effects： | Yes, in syngeneic mice |
| Comments： | The cells synthesize angiotensin-converting enzyme, express surface receptors for acetylated low density lipoprotein, produce thrombospondin and show intracellular staining with an antibody to von Willebrand factor. Cathepsin L is secreted by EOMA cells and is responsible for the generation of endostatin L. Although constitutive cytokine gene expression exists in EOMA cells, the level of IL-6 mRNA is prominently elevated by incubation with Liposome encapsulated hemoglobin (LEH). The cells constitutively express the vascular addressin identified by antibody MECA-99. EOMA cells exhibit characteristic endothelial cell properties, such as rearrangement into tubelike structures on Matrigel and retention of cobblestone morphology at confluence. They behave in vitro in a manner similar to microvascular endothelial cells. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subculture Ratio: 1:3 to 1:6 Medium Renewal: Every 2 to 3 days.
Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994 |
| Cryopreservation： | Freeze medium: Complete growth medium supplemented with 5% DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Temperature: 37°C |
| Name of Depositor： | R Auerbach |
| Deposited As： | mouse |
| Year of Origin： | 1980 |
| References： | Felbor U, et al. Secreted cathepsin L generates endostatin from collagen XVIII. EMBO J. 19: 1187-1194, 2000. PubMed: 10716919 Obeso J, et al. A hemangioendothelioma-derived cell line: its use as a model for the study of endothelial cell biology. Lab. Invest. 63: 259-269, 1990. PubMed: 2166185 Zhu XL, et al. Kinetics of cytokine gene expression in macrophage and endothelial cell lines following liposome encapsulated haemoglobin (LEH) treatment in vitro. Cytokine 8: 541-547, 1996. PubMed: 8891435 Wen W, et al. The generation of endostatin is mediated by elastase.. Cancer Res. 59: 6052-6056, 1999. PubMed: 10626789 |