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微信小章| 产品名称： | EOC 13.31 |
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| 商品货号： | TS212203 |
| Organism： | Mus musculus, mouse |
| Tissue： | brain |
| Cell Type： | microglia |
| Product Format： | frozen |
| Morphology： | macrophage |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age： | 10 days |
| Gender： | female |
| Strain： | C3H/HeJ |
| Applications： | The cells may be used to characterize the role of brain macrophages.
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| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | This is an immortalized cell line derived from the brain of an apparently normal 10 day old mouse. Ref |
| Antigen Expression： | CD11b/CD18 (Mac-1) +, Mac-2 +, Mac-3 +, CD80 (B7-1) +, CD86 (B7.2) +; CD45 +, Ly-6C +, F4/80 +, MHC Class I +, MHC Class II +, CD115 (colony stimulating factor 1 receptor (CSF-1R)) +, FcR + Ref   Walker WS, et al. Mouse microglial cell lines differing in constitutive and interferon-gamma-inducible antigen-presenting activities for naive and memory CD4+ and CD8+ T cells. J. Neuroimmunol. 63: 163-174, 1995. PubMed: 8550814 |
| Receptor Expression： | colony stimulating factor 1 (CSF-1R, CD115) |
| Comments： | The cell line is dependent on colony stimulating factor 1 (CSF-1). The cells exhibit phagocytic activity. These cells constitutively expressed high levels of major histocompatibility complex (MHC) class II antigens but unlike EOC-20 (CRL-2469) expression was not upregulated by recombinant murine interferon-gamma. C3H/HeJ strain is defective in TLR4 (toll-like receptor 4)
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| Complete Growth Medium： | Dulbeccos modified Eagles medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose, 70%; fetal bovine serum, 10%; LADMAC Conditioned Media (produced from the LADMAC cell line (CRL-2420), 20%
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| Subculturing： |
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Subculturing Procedure for LADMAC cells
Cultures can be established by centrifugation with subsequent resuspension at 2 X 10 5 viable cells/ml. Attached cells may be subcultured by tapping the sides of the flask until cells are dispersed.
Complete Growth Medium for LADMAC cells
The base medium for this cell line is ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003.xa0 To make the complete growth medium, add the following components to the base medium:xa0
This medium is formulated for use with a 5% CO2 in air atmosphere.
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| Cryopreservation： | Complete growth medium, 95%; DMSO, 5%. Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| Name of Depositor： | WS Walker |
| Deposited As： | mouse |
| Passage History： | Cells were cloned in soft agar in the presence of CSF-1 and expanded on microcarrier beads. Beads were transferred to culture dishes and were subsequently passaged by scraping. |
| References： | Olivas E, et al. Use of the Pannell-Milstein roller bottle apparatus to produce high concentrations of the CSF-1, the mouse macrophage growth factor. J. Immunol. Methods 182: 73-79, 1995. PubMed: 7769247 Walker WS. Establishment of mononuclear phagocyte cell lines. J. Immunol. Methods 174: 25-31, 1994. PubMed: 8083530 Walker WS, et al. Mouse microglial cell lines differing in constitutive and interferon-gamma-inducible antigen-presenting activities for naive and memory CD4+ and CD8+ T cells. J. Neuroimmunol. 63: 163-174, 1995. PubMed: 8550814 Askew D, Walker WS. Alloantigen presentation to naive CD8+ T cells by mouse microglia: evidence for a distinct phenotype based on expression of surface-associated and soluble costimulatory molecules. Glia 18: 118-128, 1996. PubMed: 8913775 |