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| 产品名称： | EL4.IL-2 |
|---|---|
| 商品货号： | TS212213 |
| Organism： | Mus musculus, mouse |
| Tissue： | thymus |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | lymphoma |
| Strain： | C57BL/6 |
| Storage Conditions： | liquid nitrogen vapor phase |
| Genes Expressed： | interleukin-2 (interleukin 2, IL-2) |
| Cellular Products： | interleukin-2 (interleukin 2, IL-2) |
| Comments： | This is a subline of EL4 (ATCC TIB-39) that produces IL-2 in response to phorbol-12-myristate-13-acetate (PMA). The line is capable of producing 2500 units/ml of IL-2 after 24 hours in culture with PMA. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: horse serum to a final concentration of 10%.
|
| Subculturing： | Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 105 viable cells/mL. Maintain cell density between 1 to 2 X 106 viable cells/mL.
Medium Renewal: Every 2 to 3 days |
| Cryopreservation： | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Temperature: 37°C |
| Name of Depositor： | JJ Farrar |
| Deposited As： | Mus musculus |
| References： | Farrar JJ, et al. Thymoma production of T cell growth factor (interleukin-2). J. Immunol. 125: 2555-2558, 1980. PubMed: 6968789 Cancer therapeutics. New York: Raven Press; 1981. Fuller-Farrar J, et al. Phorbol myristic acetate enhances the production of interleukin 2. Cell. Immunol. 58: 156-164, 1981. PubMed: 6784931 |