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购买须知
| 产品名称： | EL4.BU.1.OUAr.1.1 |
|---|---|
| 商品货号： | TS212214 |
| Organism： | Mus musculus, mouse |
| Cell Type： | T lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | lymphoma |
| Strain： | C57BL/6N |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | This line was derived from the EL4 cell line (see ATCC TIB-39). |
| Antigen Expression： | H-2b; Thy-1.2 |
| Comments： | This line was derived from the EL4 cell line (see ATCC TIB-39). The cells are resistant to 0.1 mM 5-bromo-2-deoxyuridine and 1 mM ouabain. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | Dulbeccos modified Eagles medium, 90%; horse serum, 10%
|
| Subculturing： | Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2-3 X 105 cells/mL and maintain between 1 X 105 and 1 X 106 cells/mL.
Medium Renewal: Every 2 to 3 days
|
| Culture Conditions： | Temperature: 37°C Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
| Name of Depositor： | R Hyman |
| Deposited As： | Mus musculus |
| References： | Hyman R, Stallings V. Characterization of a TL- variant of a homozygous TL+ mouse lymphoma. Immunogenetics 3: 75-84, 1976. |