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微信小章| 产品名称： | EL4 |
|---|---|
| 商品货号： | TS212215 |
| Organism： | Mus musculus, mouse |
| Cell Type： | T lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | lymphoma |
| Strain： | C57BL/6N |
| Applications： | This cell line is a suitable transfection host. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | modal number = 39 |
| Images： |  |
| Derivation： | EL4 was established from a lymphoma induced in a C57BL mouse by 9,10-dimethyl-1,2-benzanthracene. |
| Antigen Expression： | H-2b; Thy-1.2 |
| Comments： | The cells are resistant to 0.1 mM cortisol and sensitive to 20 μg/mL PHA.
A subline (EL4.IL-2, ATCC TIB-181) that produces high levels of interleukin-2 (IL-2, interleukin 2) is available. A subline (EL4.IL-2, ATCC TIB-40) that is resistant to 0.1 mM 5-bromo-2-deoxyuridine (BUdR) is available.
A subline (EL4.BU.1.OUAr.1.1, ATCC TIB-41) that is resistant to 0.1 mM 5-bromo-2-deoxyuridine and 1 mM ouabain is available. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: horse serum to a final concentration of 10%.
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| Subculturing： | Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 105 cells/mL and maintain between 1 X 105 and 1 X 106 cells/mL.xa0Corning® T-75 flasks (catalog #431464) are recommended for subculturing this product. Medium Renewal:xa0Every 2 to 3 days |
| Cryopreservation： | culture medium 95%; DMSO, 5% |
| Culture Conditions： | Temperature: 37°C |
| Name of Depositor： | M Cohn |
| Deposited As： | Mus musculus |
| References： | Ralph P. Retention of lymphocyte characteristics by myelomas and theta+-lymphomas: sensitivity to cortisol and phytohemagglutinin. J. Immunol. 110: 1470-1475, 1973. PubMed: 4541304 Old LJ, et al. The G (Gross) leukemia antigen. Cancer Res. 25: 813-819, 1965. PubMed: 4284252 Gorer PA. Studies in antibody response of mice to tumour inoculation. Br. J. Cancer 4: 372-379, 1950. PubMed: 14801344 Herberman RB. Serological analysis of cell surface antigens of tumors induced by murine leukemia virus. J. Natl. Cancer Inst. 48: 265-271, 1972. PubMed: 4119883 Ralph P, Nakoinz I. Inhibitory effects of lectins and lymphocyte mitogens on murine lymphomas and myelomas. J. Natl. Cancer Inst. 51: 883-890, 1973. PubMed: 4542714 Shevach EM, et al. Immunoglobulin and theta-bearing murine leukemias and lymphomas. J. Immunol. 108: 1146-1151, 1972. PubMed: 4112916 Aparicio CL, et al. Correction for label leakage in fluorimetric assays of cell adhesion. BioTechniques 23: 1056-1060, 1997. PubMed: 9421636 Cuthbert JA, Lipsky PE. Regulation of proliferation and Ras localization in transformed cells by products of mevalonate metabolism. Cancer Res. 57: 3498-3504, 1997. PubMed: 9270019 |