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| 产品名称： | EJ-6-2-Bam-6a |
|---|---|
| 商品货号： | TS212216 |
| Organism： | Mus musculus, mouse |
| Tissue： | embryo |
| Product Format： | frozen |
| Morphology： | fibroblast |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | Carcinoma |
| Age： | embryo |
| Strain： | NIH/Swiss |
| Applications： | This line was established by C. Shih et al. |
| Derivation： | This line was established by C. Shih et al. in 1981 by transfecting NIH/3T3 cells with Bam H1 digested DNA from EJ-6-2 cells (NIH/3T3 cells transformed with DNA from the human EJ bladder carcinoma EJ). |
| Tumorigenic： | Yes |
| Effects： | Yes, forms subcutaneous tumors in BALB/c and NSF mice |
| Comments： | This line was established by C. Shih et al. in 1981 by transfecting NIH/3T3 cells with Bam H1 digested DNA from EJ-6-2 cells (NIH/3T3 cells transformed with DNA from the human EJ bladder carcinoma EJ). The cells are morphologically transformed and highly tumorigenic in BALB/c and NSF mice. |
| Complete Growth Medium： | Dulbeccos modified Eagles medium with 4.5 g/L glucose; 90%; bovine calf serum, 10%
|
| Subculturing： | Subcultivation Ratio: A subcultivation ratio of 1:100 is recommended Medium Renewal: Twice per week Remove medium, add fresh 0.25% trypsin, 0.03% EDTA, rinse and remove trypsin and let the culture sit at 37C until the cells detach (5 to 10 minutes). Add fresh medium, aspirate and dispense into new flasks. Subculture twice weekly. |
| Name of Depositor： | RA Weinberg |
| Deposited As： | Mus musculus |
| Year of Origin： | 1981 |
| References： | Schechter AL, et al. The neu oncogene: an erb-B-related gene encoding a 185,000-Mr tumour antigen. Nature 312: 513-516, 1984. PubMed: 6095109 Shih C, et al. Transforming genes of carcinomas and neuroblastomas introduced into mouse fibroblasts. Nature 290: 261-264, 1981. PubMed: 7207618 |