微信小陈
微信小章| 产品名称： | EB1 |
|---|---|
| 商品货号： | TS212220 |
| Organism： | Homo sapiens, human |
| Tissue： | upper maxilla |
| Cell Type： | B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 2 Cells Contain HERPESVIRUS
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | Burkitts lymphoma |
| Age： | 9 years |
| Gender： | female |
| Ethnicity： | Black |
| Derivation： | The EB1 line was isolated in 1963 by M.A. Epstein and Y.M. Barr from biopsy fragments and cell clumps of the lymphoma. |
| Clinical Data： | female Black 9 years |
| Comments： | The EB1 line was isolated in 1963 by M.A. Epstein and Y.M. Barr from biopsy fragments and cell clumps of the lymphoma. Viral particles (Epstein-Barr virus) were noted through electron microscopic examination. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing： | Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended Medium Renewal: Twice per week Allow cells to settle, remove most of the supernatant medium, dilute with fresh medium to 4 X 10 exp5 viable cell/ml. Subculture at time of fluid renewal. |
| Cryopreservation： | Culture medium, 95%; DMSO, 5% |
| Isoenzymes： | ES-D, 1 G6PD, B GLO-I, 2 PGM1, 1 |
| Name of Depositor： | G Trempe, LJ Old |
| Deposited As： | Homo sapiens |
| References： | Epstein MA, Barr YM. Cultivation in vitro of human lymphoblasts from Burkitts malignant lymphoma. Lancet 1: 252-253, 1964. PubMed: 14090852 Epstein MA, Barr YM. Characteristics and mode of growth of tissue culture strain (EB1) of human lymphoblasts from Burkitts lymphoma. J. Natl. Cancer Inst. 34: 231-240, 1965. PubMed: 14293790 M A Epstein et al. The Behaviour and Morphology of a Second Tissue Culture Strain (EB2) of Lymphoblasts from Burkitts Lymphoma. Br. J. Cancer 19: 108-115, 1965. Giraldo G, et al. Kaposis sarcoma: a new model in the search for viruses associated with human malignancies. J. Natl. Cancer Inst. 49: 1495-1507, 1972. PubMed: 4346014 Epstein MA, et al. Morphological and biological studies on a virus in cultured lymphoblasts from Burkitts lymphoma. J. Exp. Med. 121: 761-770, 1965. Leder P, et al. Translocations among antibody genes in human cancer. Science 222: 765, 1983. PubMed: 6356357 Dalla-Favera R, et al. Human c-myc onc gene is located on the region of chromosome 8 that is translocated in Burkitt lymphoma cells. Proc. Natl. Acad. Sci. USA 79: 7824-7827, 1982. PubMed: 6961453 Taub R, et al. Translocation of the c-myc gene into the immunoglobulin heavy chain locus in human Burkitt lymphoma and murine plasmacytoma cells. Proc. Natl. Acad. Sci. USA 79: 7837-7841, 1982. PubMed: 6818551 |