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| 产品名称： | EB-3 EB3 |
|---|---|
| 商品货号： | TS212221 |
| Organism： | Homo sapiens, human |
| Cell Type： | B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 2 Cells Contain HERPESVIRUS Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | Burkitts lymphoma |
| Age： | 3 years |
| Gender： | Male |
| Ethnicity： | Black |
| Storage Conditions： | liquid nitrogen vapor phase |
| Images： |  |
| Clinical Data： | male Black 3 years |
| Antigen Expression： | HLA A3, Aw32, Cw2 |
| Virus Resistance： | partially resistant to polioviruses and vesicular stomatitis virus |
| Comments： | EBNA positive. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing： | Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively cultures can be established by centrifugation with subsequent resuspension at 3xa0x 105 viable cells/mL. |
| Cryopreservation： | culture medium, 90%; DMSO, 10% |
| Culture Conditions： | Temperature: 37.0°C Atmosphere: air, 95%.xa0carbon dioxidexa0(CO2), 5% |
| STR Profile： | Amelogenin: X,Y CSF1PO: 12,15 D13S317: 12,14 D16S539: 10,12 D5S818: 9,10 D7S820: 11 THO1: 7 TPOX: 6,9 vWA: 17,19 |
| Isoenzymes： | G6PD, A |
| Name of Depositor： | W Henle |
| Deposited As： | Homo sapiens |
| References： | Henle G, et al. Relation of Burkitts tumor-associated herpes-ytpe virus to infectious mononucleosis. Proc. Natl. Acad. Sci. USA 59: 94-101, 1968. PubMed: 5242134 Epstein MA, Barr YM. Cultivation in vitro of human lymphoblasts from Burkitts malignant lymphoma. Lancet 1: 252-253, 1964. PubMed: 14090852 |