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| 产品名称： | EH7a |
|---|---|
| 商品货号： | TS212227 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Derivation： | Animals were immunized with a mixture of Ca++ dependent phospholipid binding proteins isolated from human polymorphonuclear leukocytes. Spleen cells were fused with P3X63Ag8.653 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against human annexin I and human annexin II |
| Cellular Products： | immunoglobulin; monoclonal antibody; against human annexin I and human annexin II |
| Tumorigenic： | Yes |
| Effects： | Yes, in BALB/c mice |
| Comments： | Animals were immunized with a mixture of Ca++ dependent phospholipid binding proteins isolated from human polymorphonuclear leukocytes. Spleen cells were fused with P3X63Ag8.653 myeloma cells. The antibody recognizes human annexin I amino acids 41 - 118, and inhibits annexin I mediated phospholipid vesicle aggregation. Annexin II mediated vesicle aggregation is not affected by EH7a antibody. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
|
| Subculturing： | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Cryopreservation： | Culture medium, 95%; DMSO, 5% |
| Isotype： | IgG1 |
| Name of Depositor： | JD Ernst |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| References： | Ernst JD, et al. Identification of a domain that mediates vesicle aggregation reveals functional diversity of annexin repeats. J. Biol. Chem. 266: 6670-6673, 1991. PubMed: 1707872 |