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微信小章| 产品名称： | Duck embryo |
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| 商品货号： | TS212229 |
| Organism： | Anas platyrhynchus domesticus, duck, Pekin |
| Tissue： | embryo |
| Product Format： | frozen |
| Morphology： | fibroblast |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age： | embryo |
| Storage Conditions： | liquid nitrogen vapor phase |
| Virus Susceptibility： | Vesicular stomatitis, Orsay (Indiana)
Vesicular stomatitis, Glasgow (Indiana) Herpes simplex virus St. Louis encephalitis virus , St. Louis encephalitis virus California encephalitis virus , Melao virus Eastern equine encephalitis virus , Eastern equine encephalitis virus Anatid herpesvirus 1 Rubella virus , Rubella virus |
| Virus Resistance： | poliovirus 2 |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Volumes used in this protocol are for axa075 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. It is important to subculture the cells as soon as they become confluent, or else the cells will begin to die.
Subcultivation Ratio: 1:2 to 1:6. Medium Renewal: 1 to 2 times per week |
| Cryopreservation： | Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C |
| Name of Depositor： | M Marcovici, J Prier, M Allen |
| Deposited As： | Anas platyrhynchus domesticus |
| References： | Marcovici M, Prier JE. Enhancement of St. Louis arbovirus plaque formation by neutral red. J. Virol. 2: 178-181, 1968. PubMed: 4911850 Melnick JL. Tissue culture techniques and their application to original isolation, growth, and assay of poliomyelitis and orphan viruses. Ann. N.Y. Acad. Sci. 61: 754-772, 1955. PubMed: 13340582 Wolf K, et al. Duck viral enteritis: microtiter plate isolation and neutralization test using the duck embryo fibroblast cell line. Avian Dis. 18: 427-434, 1974. PubMed: 4368600 Buynak EB, et al. Preparation and testing of duck embryo cell culture rubella vaccine. Am. J. Dis. Child. 118: 347-354, 1969. PubMed: 4307520 Farris AD, et al. Conserved features of Y RNAs revealed by automated phylogenetic secondary structure analysis. Nucleic Acids Res. 27: 1070-1078, 1999. PubMed: 9927741 |
| Cross References： | Nucleotide (GenBank) : U82124 Anas platyrhynchus Y1 RNA, partial sequence. Nucleotide (GenBank) : U82125 Anas platyrhynchus Y3 RNA, partial sequence. |