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| 产品名称： | E.G7-OVA derivative of EL4 |
|---|---|
| 商品货号： | TS212230 |
| Organism： | Mus musculus, mouse |
| Tissue： | lymphocyte |
| Cell Type： | T lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | lymphoma |
| Strain： | C57BL/6N |
| Applications： | This cell line is a model system for studying major histocompatibility complex (MHC) class I restricted responses of cytotoxic T lymphocytes in mice. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | E.G7-OVA was derived in 1988 from the C57BL/6 (H-2 b) mouse lymphoma cell line EL4 (ATCC TIB-39).
The EL4 cells were transfected by electroporation with the plasmid pAc-neo-OVA which carries a complete copy of chicken ovalbumin (OVA) mRNA and the neomycin (G418) resistance gene.
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| Antigen Expression： | H-2 b |
| Genes Expressed： | chicken ovalbumin |
| Comments： | E.G7-OVA cells contain a single copy of the inserted plasmid and synthesize and secrete OVA constitutively.
C57BL/6 mice immunized with E.G7-OVA cells give rise to H-2 Kb restricted cytotoxic lymphocytes specific for the OVA 258-276 peptide.
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| Complete Growth Medium： | RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES and 1.0 mM sodium pyruvate and supplemented with 0.05 mM 2-mercaptoethanol and 0.4 mg/ml G418, 90%; fetal bovine serum, 10%
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| Subculturing： | Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2xa0x 105 cells/mL and maintain between 1xa0x 105 and 1xa0x 106 cells/mL. Medium Renewal: Every 2 to 3 days |
| Cryopreservation： | Culture medium, 95%; DMSO, 5% |
| Culture Conditions： | Temperature: 37°C |
| Name of Depositor： | MJ Bevan |
| Deposited As： | Mus musculus |
| Year of Origin： | 1988 |
| References： | Moore MW, et al. Introduction of soluble protein into the class I pathway of antigen processing and presentation. Cell 54: 777-785, 1988. PubMed: 3261634 |