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| 产品名称： | DU4475 |
|---|---|
| 商品货号： | TS212231 |
| Organism： | Homo sapiens, human |
| Tissue： | mammary gland; breast; derived from metastatic site: skin carcinoma |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | suspension, multicell aggregates |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | carcinoma, skin |
| Age： | 70 years |
| Gender： | female |
| Ethnicity： | Caucasian |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | modal number = 93; range = 85 to 95. The cell line is aneuploid human female, with chromosome counts in the tetraploid range. Normal chromosomes N16 and N21 are least well represented, with from none to two copies per karyotype, while chromosome N7 tends to be over-represented, with four or five copies per karyotype, whereas two to four copies are found of most of the remaining normal chromosomes. Twenty-two marker chromosomes are found including: iso(1q), del(1)(q32), der(1)t(1;?)(p36;?), del(3)(q24), der(3)t(3;?)(q24;?), del(3)(p14p25), t(6q;21?) and others. Eight are identical to those reported or portrayed in the report by A.J. Langlois, et al. |
| Images： |  |
| Clinical Data： | 70 years Caucasian female |
| Tumorigenic： | Yes |
| Effects： | Yes, in nude mice |
| Comments： | Numerous desmosomes and interdigitating microvilli were observed by transmission electron microscopy. This line grows in floating clusters and viability cannot be determined accurately with dye exclusion tests.
The cells form transplantable tumors similar in histology to that of the original carcinoma. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
|
| Subculturing： | Cultures can be maintained by addition or replacement of fresh medium. Establish new cultures at 2 X 105 cells/mL and maintain at between 105 and 106 cells/mL.
Medium Renewal: 2 to 3 times per week |
| Cryopreservation： | Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Temperature: 37°C |
| STR Profile： | Amelogenin: X CSF1PO: 9,12 D13S317: 11,14 D16S539: 11,12 D5S818: 11 D7S820: 9,10 THO1: 6,8 TPOX: 8 vWA: 17 |
| Isoenzymes： | AK-1, 1 ES-D, 1 G6PD, B GLO-I, 2 Me-2, 2 PGM1, 1-2 PGM3, 1 |
| Name of Depositor： | AJ Langlois |
| Deposited As： | Homo sapiens |
| References： | Faust JB, Meeker TC. Amplification and expression of the bcl-1 gene in human solid tumor cell lines. Cancer Res. 52: 2460-2463, 1992. PubMed: 1568216 Langlois AJ, et al. Morphological and biochemical properties of a new human breast cancer cell line. Cancer Res. 39: 2604-2613, 1979. PubMed: 221108 |