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微信小章| 产品名称： | E. Derm (NBL-6) |
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| 商品货号： | TS212232 |
| Organism： | Equus caballus, horse |
| Tissue： | skin, dermis |
| Product Format： | frozen |
| Morphology： | fibroblast |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age： | 4 years |
| Gender： | female |
| Strain： | quarterhorse |
| Storage Conditions： | liquid nitrogen vapor temperature |
| Karyotype： | model number = 64; range = 52 to 145. The donors sex (female) is confirmed chromosomally. Although the chromosome numbers vary widely, 55% of the cells have a chromosome number within + or - 1 of the diploid and modal number (64). The donors sex (female) is confirmed chromosomally. Although the chromosome numbers vary widely, 55% of the cells have a chromosome number within + or - 1 of the diploid and modal number (64). |
| Virus Resistance： | adenovirus 5; coxsackievirus A9, B5; poliovirus 2 |
| Comments： | Cells appear to senesce after 40 passages. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994. |
| Cryopreservation： | Complete growth medium, 95%; DMSO, 5%. Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C |
| Name of Depositor： | AJ Kniazeff, WA Nelson-Rees, NB Darby |
| Deposited As： | Equus caballus |
| Passage History： | Cells appear to senesce after 40 passages. |
| Year of Origin： | September, 1965 |
| References： | Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online. |