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| 产品名称： | DU1-29 |
|---|---|
| 商品货号： | TS212233 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | The antibody is specific for the sheep L-selectin molecule expressed on populations of T cells, B cells, neutrophils, medullary thymocytes and monocytes. |
| Derivation： | Spleen cells were fused with Sp2/0-Ag14 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against ovine and bovine L-selectin (CD62L) |
| Cellular Products： | immunoglobulin; monoclonal antibody; against ovine and bovine L-selectin (CD62L) |
| Comments： | Animals were immunized with CD4-, CD8- sheep thymocytes. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody is specific for the sheep L-selectin molecule expressed on populations of T cells, B cells, neutrophils, medullary thymocytes and monocytes. |
| Complete Growth Medium： | Culture Medium: Iscoves modified Dulbeccos medium, 90%; fetal bovine serum, 10%. |
| Subculturing： | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Cryopreservation： | Culture medium, 95%; DMSO, 5% |
| Isotype： | IgG1 |
| Name of Depositor： | CR Mackay |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| References： | Mackay CR, et al. Tissue-specific migration pathways by phenotypically distinct subpopulations of memory T cells. Eur. J. Immunol. 22: 887-895, 1992. PubMed: 1372559 |