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| 产品名称： | Dubca |
|---|---|
| 商品货号： | TS212234 |
| Organism： | Camelus dromedarius, camel |
| Tissue： | skin |
| Cell Type： | SV40 transformed |
| Product Format： | frozen |
| Morphology： | fibroblast |
| Culture Properties： | monlayer |
| Biosafety Level： | 2 xa0 Cells contain papovavirus
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | Autopsy |
| Age： | 2 months gestation |
| Gender： | female |
| Applications： | SV40 large T-antigen is detected by Western blotting. The cell line is susceptible to infection with viruses of several different families. The cells may be used for virus diagnosis and vaccine production. |
| Derivation： | The cell line designated Dubca (Dubai Camel) was originated in 1990 from a specimen taken from a fetus during the autopsy of the mother at the Veterinary Research Laboratory in Dubai, UAE. Cells at passage 7 were transformed by infection with SV40 and the resulting cells were cloned in soft agar. Orf, Newcastle disease virus (NDV) and Sindbis virus show an abortive infection with virus replicating only in the first passages. |
| Clinical Data： | female |
| Virus Resistance： | parapox virus; canine adenovirus type 1 (CAV); Aleutian disease virus (ADV); influenza virus (A equi 1); ECBO virus, equine arteritis virus (EAV) |
| Comments： | The cell line designated Dubca (Dubai Camel) was originated in 1990 from a specimen taken from a fetus during the autopsy of the mother at the Veterinary Research Laboratory in Dubai, UAE. Cells at passage 7 were transformed by infection with SV40 and the resulting cells were cloned in soft agar. SV40 large T-antigen is detected by Western blotting. The cell line is susceptible to infection with viruses of several different families. Orf, Newcastle disease virus (NDV) and Sindbis virus show an abortive infection with virus replicating only in the first passages. While some isolates of bovine viral diarrhea virus (BVDV) and border disease virus (BDV) could not be grown in Dubca cells other isolates could be propagated to a limited extent (only a few cells expressed viral antigen). The cells may be used for virus diagnosis and vaccine production. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended Medium Renewal: Every 2 to 3 days Remove medium, rinse with 0.25% trypsin, 0.03% EDTA solution, add an additional 1 to 2 ml of trypsin solution and allow the flask to set at room temperature (or incubate at 37C) until cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. |
| Cryopreservation： | Culture medium, 95%; DMSO, 5% |
| Name of Depositor： | OR Kaaden |
| Deposited As： | Camelus dromedarius |
| References： | Klopries M, et al. Characterization of the camel skin cell line Dubca. Br. Vet. J. 151: 555-565, 1995. PubMed: 8556315 |