微信小陈
微信小章| 产品名称： | E.H. IV (Elaine IV) |
|---|---|
| 商品货号： | TS212235 |
| Organism： | Homo sapiens, human |
| Tissue： | peripheral blood |
| Cell Type： | B lymphoblast |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 2 Cells Contain HERPESVIRUS
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | infectious mononucleosis |
| Age： | 20 years |
| Gender： | female |
| Ethnicity： | Caucasian |
| Storage Conditions： | liquid nitrogen vapor phase |
| Genes Expressed： | Epstein-Barr virus (EBV) |
| Cellular Products： | Epstein-Barr virus (EBV) |
| Virus Resistance： | partially resistant to poliovirus and vesicular stomatitis viruses |
| Comments： | The cells carry and produce Epstein-Barr virus (EBV). EBNA positive. Biological Safety CLASS II. |
| Complete Growth Medium： | RPMI 1640 medium, 80%; fetal bovine serum, 20%
|
| Subculturing： | Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively cultures can be established by centrifugation with subsequent resuspension in fresh medium at 4 to 5 x 105xa0viable cells/mL. A maximum of 1 to 2 x 106xa0cells/mL is obtainable. Medium Renewal: Add fresh medium (20% to 30% by volume) every 2 to 3 days |
| Cryopreservation： | Complete growth medium described above supplemented with 5% (v/v) DMSO. Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C Atmosphere: 5% CO2 |
| Isoenzymes： | G6PD, B |
| Name of Depositor： | W Henle |
| Deposited As： | Homo sapiens |
| References： | Henle G, et al. Relation of Burkitts tumor-associated herpes-ytpe virus to infectious mononucleosis. Proc. Natl. Acad. Sci. USA 59: 94-101, 1968. PubMed: 5242134 Niederman JC, et al. Infectious mononucleosis. Clinical manifestations in relation to EB virus antibodies. JAMA 203: 205-209, 1968. PubMed: 4864269 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |