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微信小章| 产品名称： | DS-1 |
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| 商品货号： | TS212243 |
| Organism： | Homo sapiens, human |
| Tissue： | derived from metastatic site:xa0pleural effusion |
| Cell Type： | B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | lymphangiectasia |
| Storage Conditions： | liquid nitrogen vapor phase |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Images： |  |
| Derivation： | The DS-1 cell line was established from cells in the pleural effusion from an immunodeficient patient with intestinal lymphangiectasia.
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| Antigen Expression： | HLA Class I, (100%); HLA Class II (38%); plasma cell antigen (PCA) (99%); CD2 (<5%); CD3 (<5%); CD4 -; CD5 -; CD7 -; CD8 -; CD20 -; CD30 -; CD38 -; CD45 - |
| Receptor Expression： | interleukin 6 (IL-6) |
| Genes Expressed： | surface immunoglobulin |
| Cellular Products： | surface immunoglobulin |
| Comments： | DS-1 is IL-6 dependent. |
| Complete Growth Medium： | RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate supplemented with 10 U/ml IL-6, 90%; fetal bovine serum, 10%
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| Subculturing： | Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 x 105 cells/mL. Maintain cultures between 1 x 105 and 2 x 106 cells/mL. Recovery of this line from the frozen state is improved by using a feeder layer of irradiated MRC-5 cells (ATCC 55-X).
Medium Renewal: Every 2 to 3 days |
| Cryopreservation： | Complete growth medium, 95%; DMSO, 5%. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
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| Culture Conditions： | Temperature: 37°C |
| STR Profile： | Amelogenin: X CSF1PO: 12 D13S317: 9,12 D16S539: 9,10 D5S818: 11,14 D7S820: 8,14 THO1: 6,9.3 TPOX: 9,11 vWA: 18,19 |
| Isotype： | IgG; kappa light chain |
| Name of Depositor： | The United States of America |
| U.S. Patent Number： | |
| References： | Bock GH, et al. Monoclonal antibody recognizing a surface molecule on a subset of antigen-stimulated T cells and on certain malignancies of T and B cell origin. US Patent 5,556,947 dated Sep 17 1996 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |