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微信小章| 产品名称： | DT40 |
|---|---|
| 商品货号： | TS212246 |
| Organism： | Gallus gallus, chicken |
| Tissue： | bursa |
| Cell Type： | lymphoblast |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | lymphoma |
| Age： | 1 day |
| Applications： | Transfection Host:xa0 These cells may be used for stable transfection studies. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | DT40 is an avian leukosis virus (ALV) induced bursal lymphoma cell line derived from a Hyline SC chicken.
The original lymphoma was induced by viral infection of a 1 day old chicken with Rous associated virus 1 (RAV-1).
Cell suspensions prepared from tumors that developed within the bursa of Fabricus were transferred intravenously into young syngeneic recipient chickens.
After one transfer in vivo, the DT40 cell line was established.
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| Oncogene： | c-myc + |
| Genes Expressed： | immunoglobulin; IgM (surface) |
| Cellular Products： | |
| Tumorigenic： | Yes |
| Effects： | Yes, in 2 day old syngeneic chickens; tumors were observed in the spleens, kidneys and livers of recipient animals |
| Comments： | The cell line contains proviral DNA sequences integrated upstream from the c-myc proto-oncogene and expresses increased levels of c-myc RNA.
It lacks a normal c-myc gene, but contains two copies of an ALV deregulated myc gene. The cells retain the ability to rearrange the immunoglobulin light chain gene (IgL). At the IgL locus, DT40 contains one rearranged and one germline allele. The cell line exhibits a lymphoblastoid phenotype. Infectivity assays revealed that DT40 releases low levels of infectious RAV-1. Both the c-rel gene and the v-rel oncogenes induce major histocompatibility (MHC) class II antigen expression on the DT40 cell line.xa0 The expression of MHC class II is induced more rapidly by v-rel than c-rel and several weeks after infection, rel induced class II antigen as much as 50 fold more efficiently than c-rel.xa0 |
| Complete Growth Medium： | Dulbeccos modified Eagles medium with 4 mM L-glutamine modified to contain 4.5 g/L glucose, 1.5 g/L sodium bicarbonate and 0.05 mM 2-mercaptoethanol, 75%; tryptose phosphate broth, 10%; fetal bovine serum, 10%; chicken serum, 5%
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| Subculturing： | Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 3 x 105 cells/mL and maintain between 2 x 105 and 2 x 106 cells/mL.
Medium Renewal: Every 2 to 3 days |
| Cryopreservation： | Freeze medium: Culture medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Temperature: 37°C |
| Name of Depositor： | EH Humphries |
| Deposited As： | Gallus gallus |
| References： | Hrdlickova R, et al. v-rel induces expression of three avian immunoregulatory surface receptors more efficiently than c-rel. J. Virol. 68: 308-319, 1994. PubMed: 8254742 Humphries EH, Zhang G. V-rel and C-rel modulate the expression of both bursal and non-bursal antigens on avian B-cell lymphomas. Curr. Top. Microbiol. Immunol. 182: 475-483, 1992. PubMed: 1490388 Baba TW, et al. Cell lines derived from avian lymphomas exhibit two distinct phenotypes. Virology 144: 139-151, 1985. PubMed: 2998040 Buerstedde JM, et al. Light chain gene conversion continues at high rate in an ALV-induced cell line. EMBO J. 9: 921-927, 1990. PubMed: 2155784 Kim S, et al. Ongoing diversification of the rearranged immunoglobulin light-chain gene in a bursal lymphoma cell line. Mol. Cell. Biol. 10: 3224-3231, 1990. PubMed: 2111450 Buerstedde JM, Takeda S. Increased ratio of targeted to random integration after transfection of chicken B cell lines. Cell 67: 179-188, 1991. PubMed: 1913816 |