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微信小章| 产品名称： | DoCl1 (S+L-) |
|---|---|
| 商品货号： | TS212248 |
| Organism： | Canis familiaris, dog |
| Tissue： | kidney, normal |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | sarcoma |
| Age： | adult |
| Gender： | female |
| Applications： | The cells are positive for keratin by immunoperoxidase staining. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Clinical Data： | female |
| Genes Expressed： | The cells are positive for keratin by immunoperoxidase staining. |
| Cellular Products： | keratin |
| Comments： | The cells are infected with defective Moloney sarcoma virus. The cells are positive for keratin by immunoperoxidase staining. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated McCoys 5a Medium Modified, Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: 1:2 to 1:5 Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994. |
| Cryopreservation： | Complete growth medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| Name of Depositor： | PT Peebles |
| Deposited As： | Canis familiaris |
| References： | Peeples PT, et al. Murine sarcoma virus defectiveness. Viral polymerase expression murine and nonmurine host cells transformed by S+L-type murine sarcoma virus. Virology 67: 344-355, 1975. PubMed: 52940 Peebles PT, et al. Murine sarcoma virus defectiveness: serological detection of only helper virus reverse transcriptase in sarcoma virus rescued from nonmurine S + L-cells. Virology 70: 313-323, 1976. PubMed: 57666 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |